Bisphenol-A (BPA) is one of the highest volume chemicals produced world-wide and used in the manufacture of plastics and epoxy resins that are pervasive in our environment and daily lives. The present research was carried out to investigate the effects of two different doses of Bisphenol-A (BPA) on the body weight, hematological parameters and patho-physiological changes of kidney in mice. For this study, fifteen mice, 6 to 8 weeks of age with an average bwt 27.10±0.5 gm, were randomly divided into three groups (n= 5). Group A (control) received only normal mouse pellet while group B and group C received pellet mixed with BPA @ 50 mg and 100 mg / kg bwt daily for 12 weeks, respectively. At the end of the experiment, blood and tissues were collected and processed for hematological and histopathological examination. Results showed that BPA- treated mice caused significant elevation (p<0.01) in weight gain even treated with low dose (50mg) of BPA. The mice exposed to high dose of BPA (100 mg) showed marked reduction (p<0.05) in total erythrocyte count (TEC), significant decreased (p<0.01) in hemoglobin concentration (Hb) and packed cell volume (PCV). Histopathological alterations were detected in the kidneys of BPA-treated mice. In conclusion, this study suggested that BPA exerts deleterious impacts on hematological parameters including association with renal injuries. Asian J. Med. Biol. Res. December 2020, 6(4): 635-640
Complete blood count (CBC) is used as an index of health status of human and different animals as well as to diagnose a variety of diseases. Therefore, there is a growing need of using the most suitable anticoagulant to obtain the most appropriate hemogram. The present study was designed to assess the effect of different anticoagulants viz. Heparin, Sodium Citrate and EDTA on complete blood count (CBC) in rat with a view to choosing the best suitable candidate among the common anticoagulants. A total of 30 samples out of which 10 were for each type anticoagulant were collected from 10 apparently healthy rats of Long Evans strain. From each rat 6 ml of blood was drawn and subsequently divided into three different test tubes with three different anticoagulants. The samples were analyzed for their complete blood count (TEC, TLC, Hb, Hct, DLC, absolute leukocyte count, Red Cell Indices, RDW-SD, RDWCV, Platelet, MPV, PCT and PDW) using Sysmex XT-1800i Auto hematological analyzer. Results showed a significant (p<0.05) decrease in TLC, absolute neutrophil, and platelet count, and a significant (p<0.05) higher level of relative lymphocyte count in Sodium Citrate treated sample than with Heparin and EDTA. No significant changes were observed for RBC or erythrocyte indices among the three different groups. It can be concluded that samples treated with Sodium Citrate results in significant change in blood parameters. Therefore, it is better to use Heparin and EDTA than Sodium Citrate for complete blood count (CBC). Asian J. Med. Biol. Res. March 2020, 6(1): 16-21
The performance of hematological tests deteriorates with the increase in the length of sample preservation. Therefore it has been an issue to characterize the maximum permissible period spent between blood collection and measurement to have the acceptable test report. From this view point, a study was undertaken to know about the effect of preservation length on complete blood count (CBC) in rat of Long Evans strain. A total of 30 samples were collected from 10 apparently healthy rats aged between 45-48 days and the blood samples were kept in commercial test tubes treated with EDTA. The test tubes containing whole blood samples were divided into three different groups based on preservation length and were allowed to keep at 4ºC for three different lengths of time viz. 2 hours, 4 hours and 6 hours until analysis. The samples were then analyzed for their complete blood count (TEC, TLC, Hb, PCV, DLC, Absolute Leukocyte Count, Red Cell Indices, RDW-SD, RDWCV, Platelet, MPV, PCT and PDW) using Sysmex XT-1800i auto hematological analyzer. Result showed that no significant change in CBC with the variation in preservation length. Based on these findings, it can be concluded that blood samples can be preserved for as long as 6 hours to have the same report obtainable when the samples are preserved at 4ºC in refrigerated condition for 2 or 4 hours. Asian J. Med. Biol. Res. March 2020, 6(1): 22-26
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