Defective tissue repair and remodeling are main aspects of Chronic Obstructive Pulmonary Disease (COPD) pathophysiology. Bone marrow mesenchymal stem cells (BM-MSCs) have been implicated in this direction, as their functional impairment and recruitment could possibly contribute to disease development and progression. The present study characterizes for the first time the expression of migration related chemokine receptors and their ligands in BM-MSCs from COPD patients. CXCR4/SDF1a and CCR7/CCL19-CCL21 mRNA levels were evaluated in BM-MSCs obtained from twelve COPD patients and seven healthy donors. SDF1a protein levels in sera and BM-MSCs' conditioned media were also evaluated. CXCR4, SDF1a, CCL19, and CCL21 mRNA levels were significantly reduced in COPD BM-MSCs while CCR7 levels were undetectable. Notably, SDF1a protein levels were marginally elevated in both patient sera and BM-MSCs' conditioned media while the increase in SDF1a serum levels significantly correlated with disease severity in COPD. Our findings show posttranscriptional regulation of SDF1a levels in BM-MSCs of COPD patients and significant downregulation of SDF1a and CXCR4 mRNA indicating an involvement of the SDF1a signaling pathway in the disease pathophysiology.
The world was not prepared for the global of pandemic in early 2020 with the arrival of COVID 19. Europe has some of the most developed health care systems in the world and this article explains the initial response to the pandemic from an orthopaedic and trauma viewpoint from 8 nations. Italy reported the first cluster in February, which then rapidly spread around the continent, requiring a rapid reorganization of services. The reports highlight how elective surgery was universally stopped, surgical services were reconfigured, and new practices, such as the widespread use of telemedicine, may well become permanent. It also emphasizes how the pandemic has re-educated us on the importance of a consistent and central approach to deal with a global health crisis, and how medical services need to remain flexible and responsive to new ways of working.
<p><strong>Objectives</strong>. Total Knee Replacement Surgery (TKR) is one of the most common elective orthopedic operations. Postoperative pain after total knee replacement, remains a challenge. In this retrospective observational study, we evaluated the effectiveness of 3-in-1 nerve block in patients after total knee arthroplasty compared to standard opioid treatment, and we state the reasons why this approach should still be considered.</p><p><strong>Methods</strong>. To evaluate the effectiveness of the 3-in-1 nerve block, we assessed the acute pain service archive and compared the values of the visual analog scale, by separating patients into two groups according to the analgesic regimen they received as per local protocols. In group A, patients received 0.25% bupivacaine through a 3 in 1 block catheterand additional meperidine IM if needed, while in group B they received meperidine every six hours.</p><p><strong>Results</strong>. Our analysis showed the statistically significant better effectiveness of 3-in-1 nerve block with bupivacaine administration in postoperative TKR pain control compared to repeated administration of meperidine.</p><p><strong>Conclusion</strong>. The results of our study suggest that 3-in-1 nerve block with bupivacaine is an option that must always be considered in order to alleviate post-operative pain after TKR.</p>
reamed intramedullary nailing (rin) is a surgical method of choice for treatment of diaphyseal fractures. This procedure affects the biological environment of bone tissue locally and systemically. This study investigated the influence of rin on mesenchymal stem cells (MScs) in patients with long bone fractures. The axis of c-X-c motif chemokine receptor 4 (cXcr4)/stromal cell-derived factor 1 (SdF-1) was selected since it is considered as major pathway for MSc homing and migration. iliac crest bone marrow (ic-BM) samples and blood samples were collected at two different time points. one sample was collected before the rin (Bn) and the other immediately after rin (an). BM-MScs were cultured and rT-qPcr was performed for cXcr4 mrna levels and eliSa for the SdF-1 sera levels. The experimental study revealed that there was a correlation between the increase of SdF-1 levels in peripheral blood and a decrease in the levels of cXcr4 in MScs in the ic-BM following rin. The levels of SDF-1 showed a significant increase in the sera of patients after RIN. In conclusion, the present study is the first providing evidence of the effects of rin on MSc population via the cXcr4/SdF-1 axis. The levels of serum SdF-1 factor were elevated after rin while increased levels of SdF-1 in peripheral blood were inversely correlated with the mrna levels of cXcr4 on BM-MScs after rin. Therefore, this study contributes to enlighten the systematic effects of rin on the population of MScs at a cellular level. laboratory of Molecular and cellular Pneumonology, Medical School, university of crete;
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