The He-Ne laser is generally considered as a "cold" or "soft" laser. However the present study indicates that doses from 0.3 to 0.5 J/cm2 of He-Ne laser caused intensive damage that was mainly observed on the plasma membrane and the mitochondria of the midgut cells of Drosophila auraria larvae. In contrast, doses less than 0.3 J/cm2 of the He-Ne laser didn't cause any visible effect, as measured by the electron microscope. The possible use of the He-Ne laser and the subsequent effect in relation to its application in acupuncture and therapeutic research is discussed.
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