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I. (2015), Review of methodological choices in LCA of biorefinery systems -key issues and recommendations. Biofuels, Bioprod. Bioref., 9: 606-619., which has been published in final form at http://onlinelibrary. ABSTRACTThe current trend in biomass conversion technologies is towards more efficient utilization of biomass feedstock in multi-product biorefineries. Many life cycle assessment (LCA) studies of biorefinery systems have been performed, but differ in how they use the LCA methodology. Based on a review of existing LCA standards and guidelines, this paper provides recommendations on how to handle key methodological issues when performing LCA studies of biorefinery systems. Six key issues were identified: (1) goal definition, (2) functional unit, (3) allocation of biorefinery outputs, (4) allocation of biomass feedstock, (5) land use, and (6) biogenic carbon and timing of emissions. Many of the standards and guidelines reviewed here provide only general methodological recommendations. Some make more specific methodological recommendations, but these often differ between standards. In this paper we present some clarifications (e.g. examples of research questions and suitable functional units) and methodological recommendations (e.g. on allocation).