Halal food means food that permitted under Islamic law and fulfills about requirements. The absence of information about halal food contained in UIN Sunan Ampel Surabaya (UINSA) campus area causes related research to be carried out. This study aims to determine the porcine DNA contamination on food around UINSA area using molecular technology. Twenty two samples used were foods that contain meat and may contain pork obtained from canteens around UINSA area, analyzed using Polymerase Chain Reaction (PCR) method. The analysis was started with DNA isolation of 22 food samples, electrophoresis, PCR, then visualization gel electrophoresis. Primer gene coding for cytochrome b (cyt b) which produces 149 bp of DNA fragments. The results showed that no porcine contamination in 22 food samples, while the positive control showed a band of 149 bp. End point PCR method potentially to detect porcine DNA contaminants in food products around UINSA. Therefore the food is halal and safe for consumption.
Softgel candy is soft-textured confectionery processed by the addition of several components such as gum, pectin, starch and gelatin, to obtain a supple product and packed after aging treatment first. Gelatin is one of the main components in the manufacture of soft candy derived from the hydrolysis of collagen connective tissue and animal bone that serves as the nature of gelling agents, stabilizers or emulsifiers. However, the gelatin used in products not yet labeled halal Indonesian Council of Ulama (MUI) is particularly vulnerable to pork gelatin, since pork gelatin is cheaper than cattle. The purpose of this study was to test the contaminants of pig DNA on 17 samples of soft candles not labeled halal MUI. This research used Polymerase Chain Reaction (PCR) method. Seventeen samples were isolated by DNA, then spectrophotometry was performed, followed by PCR. The PCR product is run electrophoresis. Visualize the DNA with a UV gel documentation. Primer used is primer gene encoding cyt b DNA pork. Results showed that 17 samples were negative contaminants, while the positive control of pork showed a DNA band of 149 bp. This shows that Softgel Candy 17 samples do not contain pork gelatin.
Along with the times there is an increasing need from various fields including cosmetics. The use of cosmetics among the public is used to maintain healthy skin, beautify and maintain appearance. This increased demand for cosmetics has led to industrial competition so that there are many producers who take advantage of using a mixture of harmful substances in the manufacture of cosmetics. Mercury is a toxic cosmetic ingredient which has been widely circulating in the market. The impact of using mercury includes allergies, poisoning, and damage. The halalness of a product is an important thing in the life of a Muslim, the requirements for a cosmetics are said to be halal based on the origin of the constituent ingredients, processing and the process of obtaining them, while for cosmetic safety it is based on the function and impact it causes. The importance of monitoring halal cosmetic products is not only the role of the government, but also from the producers and consumers who are wise in the use and manufacture of cosmetics. Overcoming this problem is by making cosmetics from natural ingredients in the form of jackfruit leaf extract (Artocarpus hetetophyllus) as a substitute for the use of mercury by inhibiting the formation of tyrosine which produces melanin pigment which makes skin dark. This paper uses a maceration method in extracting young jackfruit leaves, then testing the phytochemicals on the leaf extract, which contains tyrosinase inhibitors which are classified as polyphenols. Furthermore, the preparation of cosmetic gel preparations was carried out, then the stability test was carried out including the organoleptic test, pH test, viscosity test, and homogeneity test to determine the reactivity of the prepared gel which could later be mass produced. The gel making with the active compound tyrosinase inhibitor from jackfruit leaves fulfills the requirements of halal cosmetics and thoyyib is shown by not using haram and dangerous substances through the action of a tyrosinase inhibitor in the form of acetocarpanone which has no side effect on the body because it is obtained from natural ingredients.
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