To test for the effects of far‐red light on preventing budset in Picea abies, seedlings of six populations originating from latitudes between 67°N and 47°N were grown for 4–8 weeks in continuous incandescent (metal halogen) light at 300 µmol m−2 s−1 and 20°C and then transferred, at the same temperature, to a daily regime of 8 h incandescent light (300 µmol m−2 s−1) followed by 16 h cool white fluorescent light (40 µmol m−2 s−1). (Cool white lamps are deficient in far‐red light, with a R/FR ratio of 7.5 compared with 2.0 for the incandescent lamps.) All the seedlings from 67° and 80% of those from 64° stopped extension growth and set terminal buds within 28 days of the change of regime. The seedlings from 61° and further south continued growing, as did control seedlings from 67° grown as above but with incandescent light at 20 µmol m−2 s−1 replacing cool white illumination. To distinguish between a clinal and ecotypic pattern of variation, the interval between 64° and 59° was investigated by growing populations originating from that area in the same regimes as before. After 28 days in the cool white day‐extension regime, the percentage budset was 86 for the population from 64°, 0 for the population from 59° and 25–50 for the intermediate populations; i.e. the populations showed a clinal variation in requirement for far‐red light according to latitude. Thus northern populations of Picea abies appear to behave as ‘light‐dominant’ plants for the photoperiodic control of extension growth and budset, whereas the more southern populations behave as ‘dark‐dominant’ plants.
In Picea abies seedlings the critical night length for bud‐set was determined for provenances from different latitudes, longitudes and altitudes within the natural range of the species. The clinal variation of this character was demonstrated. Inheritance studies indicated that this character is controlled by many genes with predominantly additive effects. In seedlings of Pinus sylvestris and Pinus contorta, growth cessation and bud‐set took place in all light regimes, thus, even under continuous illumination. A photoperiodic optimum for height growth was determined. The photoperiodic influence on such characters as recurrent flushing of shoots, needle growth, dry matter production and frost resistance was demonstrated for northern and southern populations of the two Pinus species. Shorter nights were needed to induce a particular photoperiodic response in the northern populations as compared with those from the south. The importance of reliable phenological characters for assessing frost hardiness in provenance and progeny trials by means of early tests, is discussed.
Identical pair crosses, including reciprocals, in Pinussylvestris L. (Scots pine) were made on ramets of the same clones in three clonal archives (seed orchards) in Sweden: Sävar (64°N), Röskär (59.5°N), and Degeberga (56°N). The offspring were used to test the hypothesis that the parental environment could affect the performance of the progeny (aftereffects). Growth and freezing tests were performed in the controlled conditions of the Stockholm Phytotron. Parental environment affected seed weight: the heaviest seeds came from Röskär and the lightest seeds, from Degeberga. Height development was affected in the two growth periods tested: seeds from Sävar produced the shortest plants and seeds from Röskär, the tallest plants. There was an effect on the autumn frost hardiness in the first growth period that disappeared after the second growth period. The most hardy progenies came from Sävar. The aftereffects of the parental environment were less than the maternal effects on seed weight and also less than the effects of full-sib families on growth and autumn frost hardiness. Small but mostly significant reciprocal effects were found for height and height increment during the second growth period. There was a significant reciprocal effect for seed weight. Seed weight differences could explain only a small part of the effects on growth and none of the effects on hardiness.
Diallel crosses were made between clones of a French and a Swedish provenance of Picea abies. The former is characterized by a long critical night length for bud-set and a late flushing of the buds; the latter by a short critical night length for bud-set and an early flushing of the buds. The F1 hybrid seedlings and their French and Swedish intraprovenance half-sibs were tested over three growth periods in the phytotron at the College of Forestry, Stockholm. In comparison with intraprovenance half-sibs, the hybrid progenies gave, on average, an intermediate response for the photoperiodic control over budset and for the temperature requirements for bud-flushing. This indicates the prevalence of additive action of multiple factors in the determination of the photoperiodic and temperature response. However, individual interprovenance hybrid progenies revealed a range of responses, and in certain combinations the response of these hybrids and their intraprovenance half-sibs coincided. By selection of suitable parents, interprovenance crosses can be used to produce hybrids with desired photoperiodic characteristics and temperature requirements.
Abseisic acid content at defined levels of bud dormancy and frost tolerance in two contrasting populations of Picea abies grown in a phytotron -Physiol Plant 87: 203-210.Seedlings of a southern (Romanian) and a northern (Swedish) population of Picea abies were cultivated under continuous light and 20°C for 10 weeks. To arrest growth, induce terminal bud dormancy and promote frost tolerance the seedlings were then exposed to 16 h nights for 12 weeks, with gradually lower temperature during the last 6 weeks. Samples for estimating the abscisic acid content of the needles were taken just before the onset of the night treatment, at day 3 of the treatment, and then with one, and later 2 week, intervals. From the second week onwards (third week for frost tolerance) bud dormancy and frost tolerance were assessed at the same time as abscisic acid (ABA) determinations. Phosphate-buffered saline extracts were purified on mini-columns (in some eases immunoaffinity colums) and quantified by HPLC. The degree of dormancy was estimated by transferring the seedlings to growth conditions and determining the number of days until growth was resumed. The frost tolerance of the needles exposed to -10°C and -20°C was classified in 6 classes. The frost tolerance of the terminal buds was estimated as the number of seedlings that showed some growth after 6 weeks in growth conditions. The night treatment rapidly induced terminal bud dormancy in both populations, but the release of dormancy occurred earlier in the northern population. The needles and the terminal buds became highly frost tolerant more rapidly in the northern than in the southern population and before the temperature decrease. TTie degree of dormancy began to decline before full frost tolerance was obtained in the southern population and this decline continued in both populations, while frost tolerance remained at a high level. The southern population showed a transient peak in ABA content at day 3. Although the ABA content of the northern population was lower than in the southern before the 16-h night treatment, it increased in the northern population during the treatment period, in particular after the temperature decrease. latitig frost hardiness could be identified. One way to approach the identification of these genes may he to Frost hardiness is of decisive itnportance for the sur-identify the components of hardiness. Another may he vival, growth and reprodtiction of Scandinavian forest to study the steps from the perception of the signal from trees. Breeding would he facilitated if the genes regu-the environment up to the time when the highest degree
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