ObjectivesComplete and accurate human skeletal inventory is seldom possible in archaeological and forensic cases involving severe fragmentation. In such cases, skeletal mass comparisons with published references may be used as an alternative to assess skeletal completeness but they are too general for a case-by-case routine analysis. The objective is to solve this issue by creating linear regression equations to estimate the total mass of a skeleton based on the mass of individual bones. Material and MethodsTotal adult skeletal mass and individual mass of the clavicle, humerus, femur, patella, carpal, metacarpal, tarsal and metatarsal bones were recorded in a sample of 60 skeletons from the 21 st century identified skeletal collection (University of Coimbra). The sample included 32 females and 28 males with ages ranging from 31 to 96 years old (mean = 76.4; sd = 14.8). Skeletal mass linear regression equations were calculated based on this sample. ResultsThe mass of individual bones was successfully used to predict the approximate total mass of the adult skeleton. The femur, humerus, and second metacarpal were the best predictors of total skeletal mass with root mean squared errors ranging from 292.9 to 346.1 gm. DiscussionLinear regression was relatively successful at estimating adult skeletal mass. The non-normal distribution of the sample in terms of mass may have reduced the predictive power of the equations. These results have clear impact for bioanthropology, especially forensic anthropology, since this method may provide better estimates of the completeness of the skeleton or the minimum number of individuals.Keywords: bioarchaeology; forensic anthropology; bone mass; scattered remains; funerary practice.The objective of this paper is to investigate the potential of linear regression to estimate the mass of human adult skeletons based on the mass of individual bones. In some cases involving skeletal remains, it may be difficult to assess how complete the skeleton is due to fragmentation that prevents the anatomical identification of all skeletal elements. For example, it may be difficult to estimate the minimum number of individuals (MNI) or decide when to conclude forensic searches for the remains of victims when it is impossible to determine the amount of missing bones, especially if the remains are very fragmented, commingled and/or scattered. Such inventory problems are often more complicated still in cases involving burned skeletal remains. In the case of archaeological cremations, an exhaustive inventory is often impossible to accomplish due to the high number of anatomically unidentified fragments (Gonçalves et al, 2015). Therefore, unorthodox methods to assess skeletal completeness are worth exploration.To our knowledge, the only alternative method to assess skeleton completeness is by weighing remains to provide an estimate of skeletal mass. This is then compared with references obtained from samples of complete adult skeletons (e.g. Ingalls, 1931;Lowrance and Latimer, 1957;Silva et al.,...

Age at death estimation in burnt human remains is problematic due to the severe heat-induced modifications that may affect the skeleton after a burning event. The objective of this paper was to assess if cementochronology, which focuses on the cementum incremental lines, is a reliable method of age estimation in burnt remains. Besides the classical approach based on the counting of incremental lines, another approach based on the extrapolation of incremental lines taking into account the cement layer thickness and the incremental line thickness was investigated. A comparison of the performance of the two techniques was carried out on a sample of 60 identified monoradicular teeth that were recently extracted at dentist offices and then experimentally burnt at two maximum temperatures (400 and 900 °C

Sex diagnosis is a crucial element in the analysis of skeletal remains from forensic and archaeological contexts. Thus, researchers have developed several methods using different anatomical regions to estimate sex. Despite such variety of methods, sexing of collective cremated human skeletal remains is still challenging due to heatinduced size changes and fragmentation, along with the typical commingling of collective contexts. This study aims to examine the potential of burned tooth crowns for odontometric sex estimation. To that end, heat-induced size changes were quantified in experimentally burned teeth. Then, odontometric sex estimation was performed in a set of theoretical samples of pre and post-burned tooth crowns. Results show burned tooth crowns undergo variable but consistent and statistically significant expansion, which is due to micro-fracturing. Such heat induced size changes are of sufficient magnitude to impact odontometric sex diagnosis and sex ratios of the theoretical samples. Yet, sexing using burned tooth crowns may still be useful to estimate the minimum number of females in a given sample. Further, the effect of heat-induced size changes may be calculated and removed using scanning.

In very fragmentary remains, the thorough inventory of skeletal elements is often impossible to accomplish. Mass has been used instead to assess the completeness of the skeleton. Two different mass-based methods of assessing skeleton completeness were tested on a sample of experimentally burned skeletons with the objective of determining which of them is more reliable. The first method was based on a simple comparison of the mass of each individual skeleton with previously published mass references. The second method was based on mass linear regressions from individual bones to estimate complete skeleton mass. The clavicle, humerus, femur, patella, metacarpal, metatarsal and tarsal bones were used. The sample was composed of 20 experimentally burned skeletons from 10 males and 10 females with ages-at-death between 68 and 90years old. Results demonstrated that the regression approach is more objective and more reliable than the reference comparison approach even though not all bones provided satisfactory estimations of the complete skeleton mass. The femur, humerus and patella provided the best performances among the individual bones. The estimations based on the latter had root mean squared errors (RMSE) smaller than 300g. Results demonstrated that the regression approach is quite promising although the patella was the only reasonable predictor expected to survive sufficiently intact to a burning event at high temperatures. The mass comparison approach has the advantage of not depending on the preservation of individual bones. Whenever bones are intact though, the application of mass regressions should be preferentially used because it is less subjective.

The diagnostic accuracy of the I 3M to assess the legal age of 18 years has already been tested in several specific-population samples. The left lower third molar has been extensively used for discriminating between minors and adults. This research aimed to compare the usefulness of lower third molar maturity indexes, from both left and right side (I 3M L and I 3M R), in samples originating from four distinct continents in order to examine possible differences in their accuracy values. For this purpose, a sample of 10,181 orthopantomograms (OPGs), from Europe, Africa, Asia and America, was analysed and previously scored in other studies. The samples included healthy subjects with no systemic disorders with both third molars and clear depicted root apices. Wilcoxon Signed Rank test for left and right asymmetry did not show any significant differences. Data about sensitivity, specificity, predictive values, likelihood ratio and accuracy were pooled together and showed similar results for I 3M L and I 3M R, respectively. In addition, all these quantities were high when only the I 3M R was considered to discriminate between adults and minors. The present referable database was the first to pool third molar measurements using panoramic radiographs of subjects coming from different continents. The results highlighted that both I 3M L and I 3M R are reliable indicators for assessing the legal age of 18 years old in those jurisdictions where this legal threshold has been set as the age of majority.

Access to better health care anticipates that more medical devices can be found alongside skeletal remains. Those employed in oral rehabilitation, with available brands or batch/series, can prove useful in the identification process. A previous study in the Colecção de Esqueletos Identificados Século XXI described macroscopically the dental prostheses. An unusual case of a dental device with chromatic alterations demonstrated to require a more detailed analysis. The individual, a 53-year-old male, exhibited, at both arches, a fixed tooth-supported rehabilitation, with gold colouring classified initially as a gold-palladium alloy. Simultaneously, a green pigmentation deposit was observable in bone and prosthesis. This investigation aimed to verify the elemental composition of the dental prosthesis alloy. Elemental analysis was performed by X-ray fluorescence in two regions (labial surface of the prosthetic crown and the root surface of the lower right lateral incisor). Both the spectra and the qualitative results found higher levels of copper and aluminium, followed by nickel, iron, zinc, and manganese. No gold or palladium was detected. The most probable assumption is that a copper-aluminium alloy was used, as its elemental concentration corresponds to those measured in similar devices. Dental prostheses of copper-aluminium alloys have been made popular since the 1980s, particularly in the USA, Japan, and Eastern Europe. Apart from the biographical information, it was also known that the individual’s place of birth was an Eastern European country, which highlighted the usefulness of this type of information when dealing with missing people cases.

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