The natural benzoquinone, embelin, from the Embelia species has therapeutic benefits in a wide range of diseases. Although several extraction methods and solvents have been explored, consensus on the economic use of material and time was ambiguous. The purpose of this study was to devise a protocol for the rapid estimation of embelin. Chloroform, ethyl acetate and acetone extracts were prepared using soxhlet, microwave, sonication and cold extraction methods. The bioactivity of the chloroform extracts was assayed using the DPPH radical scavenging and the Reducing Power Assays. The embelin content in chloroform and ethyl acetate extracts were better in some extraction methods. The chloroform extracts exhibited antioxidant activity which remained unaffected regardless of the extraction technique. The microwave extraction technique yielded quick and accurate results. This technique could be adopted for rapid screening of samples with limited availability of biomass.
Garcinia mangostana is a tropical tree found across Southeast Asia, Australia and Kerala (India). The fruit has several therapeutic properties and its rind in particular has anti-microbial, anti-malarial, antioxidant and larvicidal activity. The protocol for toxicity was followed as per the OECD guidelines number: 202 and observations were recorded after 24 h and 48 h. The dose-response graph was plotted to determine the EC 50 values.The EC 50 value of the cold extract, Soxhlet extract and microwave assisted extract was found to be 0.51 ppm, 0.49 ppm and 0.83 ppm respectively.
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