We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these insects. However, we have now identified a polysaccharide from Bombyx mori that activates innate immunity in RAW264 cells and penaeid prawns. This purified polysaccharide, termed silkrose of B. mori (silkrose-BM), has a molecular weight of 1,150,000 and produces a single symmetrical peak on HPLC. Eight of nine constitutive monosaccharides of silkrose-BM are concomitant with dipterose of B. cucurbitae (dipterose-BC) and silkrose of A. yamamai (silkrose-AY). The major differences are found in the molar ratios of the monosaccharides. Silkrose-BM is approximately 500-fold less potent than silkrose-AY (EC50: 2.5 and 0.0043 μg/mL, respectively) in a nitrite oxide (NO) production assay using RAW264 cells. However, the maximum NO production for silkrose-BM and AY were comparable and higher than that of the lipopolysaccharide of Escherichia coli. The survival of penaeid prawns (Litopenaeus vannamei and Marsupenaeus japonicus) after infection with Vibrio penaecida was significantly improved by both dietary silkrose-BM and B. mori pupae. This suggests that silkrose-BM effectively prevents vibriosis in penaeid prawns via the activation of innate immunity.

Orange-spotted grouper is one of the coral reef fish has the economic value and exploited by local fisherman. Information about the size structure and sex ratio are urgent to formulate a policy for sustainability. This research aims to analyze the size structure and sex ratio of the orange-spotted grouper in Kwandang Bay. The research was conducted from December 2016 to November 2017. Sampling is carried out twice a month for one year. The total sample of orange-spotted grouper used for the analysis of the sex ratio was 149 individuals. Sample of orange-spotted grouper collected from fish landed and middlemen (grouper traders) at the Kwandang Fishing Port. Data analysis applying chi-square. The results show that males bigger than females. The sex ratio of orange-spotted grouper is 87.25 % female, 7.38 % male, and 5.37 % hermaphrodites. Orange-spotted grouper dominated by females and undergoes a gonad differentiation to male (protogynous hermaphrodite). It is concluded that The size of the male orange-spotted grouper is larger than that of the female grouper. The caught orange-spotted grouper is dominated by the female phase and undergoes a differentiation of the gonad from female to male (protogynous hermaphrodite)