Bagridae is one of the important Background: Hemibagrus wyckii economic fish species that lives in the rivers and reservoir in Riau Province, Indonesia. The present study aimed to determine the reproductive performance of under culture conditions. H.wyckii : A total of 10 female and 10 male fish were selected, and weight, Method s length, characteristics of egg and sperm, and hatchery performance were measured. Eggs were fertilized using the dry method. Egg weight and egg diameters were measured for 50 eggs per female. Egg size (50 eggs for each fish) was measured using Labo microscope model L-711 using software camera 3. Then, saline solution was added over the eggs, followed by the addition of pooled sperm from 10 males.Average relative fecundity, egg weight and egg diameter were Results: 2060±512 eggs/kg fish, 29.86±1.21 mg and 2.67±0.26 mm, respectively. The fertilization rate and hatching rate were 60.91±4.68% and 42.91±2.92% respectively. Sperm characteristics such as volume per fish (mL), pH, concentration (per mL), motility (%) and duration of motility (second) were 0.82±0.20, 7.15±0.12, 3.68±0.15, 72.77±1.46 and 47.5±4.84, respectively.The study results and scientific observations regarding Conclusion: reproductive performance suggest that can be considered a new H. wyckii candidate species for aquaculture.

Background: Giant gourami (Osphronemus goramy Lacepede, 1801) has become popular aquaculture in Indonesia. However, information on the feed used is minimal. This study analyzed the change in feeding nutrition, fish growth, feed efficiency, and body-carcass using product formulated from water coconut, palm sap sugar, and fungus. Methods: A total of 2,000 ml coconut water and 1,000 ml of palm sap sugar solution were formulated. Each product formulated was added with 6 g of Aspergillus niger (called product P1), 6 g of Rhizopus oligosporus (called product P2), and 6 g of Saccharomyces cerevisiae (called product P3). Commercial feeds supplemented with P1, P2, and P3 products are designated P1, P2, and P3 diets. Commercial feed added to freshwater is called the P4 diet (placebo). Their dosage is 300 ml/kg of feed. Gurami sago juveniles (initial weight 50±2.5 g and length 13.2±0.4 cm) were stocked in triplicate nets (2×1×1 m) in a freshwater concrete pond with a stocking density of 30 fish/net, an initial feeding rate of 3% per day until study termination. Results: Our results support our hypothesis that different product formulations have a significant effect (p<0.05) on growth performance and feed efficiency. At the same time, supplementing commercial feed with varying formula products has a significant impact (p<0.05) on the fatty acid composition of the diets and carcass body of gurami sago. Conclusion: Diet P2 contains a higher concentration of fatty acids to increase body weight, feed efficiency, and the best carcass fatty acid composition than other experiments for sago gurami reared in freshwater concrete ponds.

The utilization of genetically modified soybean meal (GM SBM) was compared with that of non-GM SBM in Nile tilapia. Four experimental diets were formulated to include either non-GM or GM SBM at 34 or 48%, respectively. These diets were fed to juvenile Nile tilapia (49.5 g average weight) for 12 weeks. The uptake of the cauliflower mosaic virus 35S promoter fragment of the GM SBM in fish muscle was examined at 8th and 12th week. After 12th week, fish were fed the non-GM SBM diets to determine the residual span of the incorporated promoter fragment. There was no significant difference in specific growth rate or feed efficiency between GM and non-GM groups at the same inclusion level. A small number of muscles from fish receiving both levels of GM SBM diet were positive for the promoter fragment. Additionally, the promoter fragment was not detected by the second day after changing to the non-GM SBM diets. These results indicate that the utilization of GM SBM was similar to that of non-GM SBM and the promoter fragment was rarely found in fish muscles, suggesting that suitability and safety of GM SBM in Nile tilapia diet were similar to those of non-GM SBM.

Background: High stocking density and intensive feeding in aquaculture systems lead to the accumulation of organic waste, which results in an increase in ammonia, nitrite, and nitrite concentrations in culture media. Biofloc is a potential technology to overcome this problem. The starter is a crucial carbon source for bacteria in the formation of biofloc. The objective of the present study aimed to explore the best starter of biofloc in a red tilapia Oreochromis niloticus culture system. Methods: A completely randomized design with four levels of treatment was used in this study. The tested starter was (A) control treatment, biofloc without starter, (B) biofloc with molasses starter, (C) biofloc with tapioca starter, and (D) biofloc with sucrose starter. The floc was cultured in 100-L tanks with a salinity of 17 ppt. The tanks were stocked with O. niloticus with a size of 3.71±0.11 cm at a stocking density of 30 fish per tank. The fish were fed on a commercial diet two times a day at satiation for 40 days. The ammonia, nitrite, and nitrite concentrations were measured for an interval of 8 days. Results: The study showed that the NH3-N range was 0.02–0.07 mg L−1 (mean, 0.03 ± 0.02 mg L−1), NO2-N range was 0.20–0.43 mg L−1 (mean, 0.25 ± 0.12 mg L−1), and NO3-N range was 0.90–3.20 mg L−1 (mean, 1.42 ± 1.19 mg L−1). Conclusion: Among the starters tested, molasses was found to be the best for biofloc in tilapia culture.