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Maha Pancha Gavya (MPG), a concoction made from five cow products was tested for its toxicity against Pythium aphanidermatum (Edson) Fitz. and its antagonists at 5, 10, 25, and 50% concentration in in vitro to find out if it can be used in integration for the control of damping-off in tomato in nursery beds. MPG was very effective inihibiting the growth of P. aphanidermatum. At the highest concentration, the growth of the pathogen was negligible. Isolates of Trichoderma viride, T harzianum, and T. virens were also sensitive to MPG at all the concentrations. Their radial growth decreased, but it was fluffy in nature and sporulated profusely. MPG was not toxic against two bacterial antagonists i.e., Bacillus subtilis and Pseudomonas fluorescens. Soil application of 10% MPG to nursery beds improved seedling stand and gave upto 48.27% tlisease control, which was more than that given by individual antagonists. However, MPG improved disease control efficacy of all the antagonists when it was used in combination with them. Integrated treatment with MPG and B. subtilis gave maximum disease control (65.33%). MPG enhanced seedling growth and it was more in combination with T. viride and B. subtilis. MPG in integration with neem cake and neem leaf extract gave complete control of damping-off and maxium increase in height of the tomato seedlings.

SUMMARYThe presence of different carbon and nitrogen sources and bivalent metal compounds in the substrate medium influenced the mycelial growth of Macrophomina phaseolina and in vitro susceptibility to fungicides. The inoculum from such substrate media showed differences in pathogenicity on mung bean (Vigna radiata). Sucrose and asparagine significantly increased the mycelial growth as well as pathogenicity of the fungus. Absence of bivalent metal ions, viz., Fe++, Zn++ and Mg++ in the medium produced inoculum which caused maximum seedling mortality and foliage blight.Carbendazim and thiophanate‐M as seed treatments were significantly less effective when the inoculum was from a medium containing glucose than when the inoculum was from a medium containing sucrose. Captafol and thiram gave significantly better disease control on mung bean when the inoculum used for soil inoculations was from media containing asparagine and ammonium nitrate compared to the inoculum grown on a medium containing sodium nitrate. Carbendazim, thiophanate‐M, PMA, captafol and thiram gave good disease control when the inoculum used was raised on a medium devoid of bivalent metal ions. Carbendazim and thiophanate‐M were the best fungicides as foliar treatments and controlled the disease irrespective of carbon, nitrogen and bivalent metal ion status of the substrate medium used for the production of inoculum.

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