Silver nanoparticles Ag-NPs have potent biological properties such as antibacterial, antifungal, anti-viral and anti-inflammatory activities. A simple, eco-friendly, inexpensive method was conducted in order to synthesize silver nanoparticles by actinomycetes. A total of 17 actinomycetes isolates were recovered from serially diluted compost sample and subsequently examined for their capabilities to produce Ag-NPs. Alongside, Candida albicans was isolated from human semen that affect male infertility, and subsequently submitted to susceptibility test using the well diffusion method. It was found that the Ag-NPs biosynthesized by Streptomyces griseus Azhar.S2 have high zone of inhibition against Candida albicans which was recorded by measuring the diameter of inhibition zone that was 20.5 ± .05 mm. The biosynthesized Ag-NPs had been characterized using UV-vis spectroscopy, FTIR, EDX and TEM. Conclusively, Candida albicans isolated from different types of dysspermatism human semen was highly eradicated by microbially synthesized Ag-NPs from Streptomyces griseus Azhar.S2.
Background:
The most significant sexually transmissible fungal disease, semen
candidiasis, is caused by Candida albicans and impacts male reproductive potential. Actinomycetes are a group of microorganisms that could be isolated from various habitats and
used for the biosynthesis of various nanoparticles with biomedical applications.
Objective:
Testing antifungal activity of biosynthesized Ag nanoparticles versus isolated
C. albicans from semen as well as its anticancer activity versus the Caco-2 cell line.
Methods:
Screening 17 isolated actinomycetes for the biosynthesis of Ag nanoparticle biosynthesis. Characterization of biosynthesized nanoparticles, testing its anti-Candida albicans, and antitumor activity
Results:
Streptomyces griseus was the isolate that identified silver nanoparticles using UV,
FTIR, XRD and TEM. Biosynthesized nanoparticles have promising anti-Candida albicans with MIC (125 ± 0.8) µg/ml and accelerate apoptotic rate versus Caco-2 cells (IC50 =
7.30 ± 0.54 µg/ml) with minimal toxicity (CC50 = 142.74 ± 4.71 µg/ml) versus Vero cells.
Conclusion:
Certain actinomycetes could be used for the biosynthesis of nanoparticles
with successive antifungal and anticancer activity to be verified by in vivo studies.
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