The effect of difiusion on the pH and temperature profiles of immobilized enzymes has been quantitatively assessed using the theory of porediKusion. The pH profiles of immobilized enzyme systems varied from those of soluble ones to the square root of the relative values of the soluble ones depending on the degree of pore difiusion limitation. Also the decrease in activation energy was predicted quantitatively with considerable accuracy in terms of eKectiveness factor. The experimental pH profiles of a few immobilized enzymes were compared with those theoretically predicted.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.