SummaryThe present investigation on effect of different growth regulator combinations on the per cent media browning in walnut in vitro studies using MS medium was carried out in order to document the available genetic variability in walnut germplasm and to select elite walnut genotypes possessing superior attributes and quality traits. During the survey, data was recorded on one hundred fifty two (152) walnut trees growing in different areas of Kashmir valley. The study also involved establishment of response of elite walnut selections to different plant growth regulators in shoot morphogenesis. Woody species have been found to be far more difficult to clone in vitro than herbaceous plants. Poor response of the explants from mature woody species to in vitro manipulation is usually associated with the problem of browning and explant necrosis. The present studies were conducted on forced explants from three walnut selections (SKUAST 002, SKUAST 008, SKUAST 010). Murashiage and Skoog's basal medium supplemented with 0.3 mg/l -1 Benzylamino purine and 0.1 mg/l -1 indole-3-butyric acid gave best response in the establishment of initiating cultures, minimum media browning (80.44 %), minimum explant browning (78.22 %) and minimum mean browning score per explant (9.17 %). The survival (23.45%) and growth of the cultures (21.77%) was also found to be maximum in MS medium supplemented with BAP 0.3 mg l -1 and IBA 0.1 mg l -1 .
:The present investigation entitled estimation of variability and genetic parameters for nut characters (weight, diameter and length) in the seedling raised natural walnut (Juglans regia L.) population in the Kashmir valley was carried out in order to document the available genetic variability in walnut germplasm and to select elite walnut genotypes possessing superior attributes and quality traits. During the survey, data was recorded on one hundred fifty two (152) walnut trees growing in different areas of Kashmir valley. Remarkable variability was observed in seedling walnut trees for different morphological, nut and kernel characters. Similarly, variations were also reported for other characters viz., tree vigour, growth habit, branching habit, leaflet shape, shoot colour, nut shape, shell texture, shell colour, shell seal, shell strength, shell integrity, kernel shrivel and kernel colour. studied the nut length varied from 30.0-60.0 mm with a population mean of 55.62 ± 3.28mm. The phenotypic and genotypic variance was 605.14 and 427.56, corresponding to their co-efficients of variation to the extent of 44.25 and 37.17 per cent, respectively. Heritability (broad sense) and expected gain (% of the mean) was 70.65 per cent and 64.36, respectively. Nut weight revealed a population mean of 14.48 ± 2.54 g with a range of 8.50-30.0 g. The phenotypic (18.785) and genotypic (12.760) variance when translated into their respective co-efficients of variation gave the estimate of 27.98 and 23.02 per cent, respectively. Broad sense heritability was 67.9 per cent and expected genetic gain (% of the mean) as 39.15.
KEY WORDS :
The present investigation on estimation of variability and genetic parameters for kernel quality traits (protein and oil content) in the seedling raised natural walnut (Juglans regia L.) population in the Kashmir valley was carried out in order to document the available genetic variability in walnut germplasm and to select elite walnut genotypes possessing superior attributes and quality traits. During the survey, data were recorded on one hundred fifty two (152) walnut trees growing in different areas of Kashmir valley. Remarkable variability were observed in seedling walnut trees for different morphological, nut and kernel characters. Similarly, variations were also reported for other characters viz., tree vigour, growth habit, branching habit, leaflet shape, shoot colour, nut shape, shell texture, shell colour, shell seal, shell strength, shell integrity, kernel shrivel and kernel colour. Studies that the oil content of kernels had a population mean of 62.102 per cent with a range of 50.22-70.00 per cent. Maximum kernel oil content (70%) was found in the walnut selection WS-114 and minimum (50.22%) in the WS-150. Analysis of variance revealed a phenotypic variance of 29.027 as compared to genotypic variance of 8.546 with environmental variance being 20.48. Based on the estimation of components of variance, the phenotypic and genotypic co-efficients of variation were 8.67 and 4.70 per cent, respectively. Heritability (broad sense) was 47.43 per cent and the expected genetic gain as only 5.261 (% of the mean).
Key Words : Walnut, Kernel characteritcies, Oilcontent, ProtinesHow to cite this article : Lone, Imtiyaz Ahmad (2017). Estimation of variability and genetic parameters for kernel quality traits (protein and oil content) in the seedling raised natural walnut (Juglans regia L.) population in the Kashmir valley.Internat. J. Plant Sci., 12 (2): 258-261,
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