With no predicted indication of adverse effects on humans, this study unravels four active multi-target inhibitors against AChE with promising affinities and good ADMET profile for the potential use in AD treatment.
The antibacterial effects of Artemisia fragrans essential oil were studied in this research. The composition of essential oil from aerial parts was analyzed by GC/MS and its antibacterial effects were determined by disk diffusion method. In this study the antibacterial effects of essential oil of A. fragrans on two types of Gram positive bacteria Staphylococcus aureus, Strep. Enterococcus fecalis and Gram negative bacteria; Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa and Klebsiella pneumonia by disk diffusion method on Mueller Hinton agar medium, were evaluated. The oil in concentrations 0, 10, 25, 50, 75, 100 percent prepared in DMSO and then 50 mg ml -1 A. fragrans essential oil was each was added to the specified disks. The present study describes the phytochemical profile and antibacterial activity of essential oil of A. fragrans. The antibacterial test results showed that the oil had a potential antibacterial activity against Gram positive bacterial strains that were mentioned. Essential oil showed maximum zone of inhibition concentration against Escherichia coli and Klebsiella pneumonia and minimal inhibition concentration against other Gram-negative bacteria that were studied.
Bovine casein solution was hydrolysed by trypsin for 4 h at a ratio of 1:100. Throughout the experiment, the solution temperature and pH were maintained at 37°C and 7.5, respectively.
AbstractNowadays, the need for new dietary containing some bioactive peptides with specific amino acid sequences increased to express biological functions. We investigated the hypocholesterolemic effect and ACE-inhibiting activity of bovine casein hydrolysates with different molecular weights. The bovine casein was hydrolysed with trypsin for different amounts of time to release bioactive peptides from the casein. Then, crude casein hydrolysate was ultrafiltered using membranes with two different molecular-weight cut offs (1 kDa and 10k Da) to determine the hypocholesterolemic effects and ACE-inhibiting activities of peptides with different molecular weights. The results showed that a higher level of hydrolysis (more active bioactive peptides) was associated with greater hypocholesterolemic and ACE-inhibiting effects in all the tested samples. The unhydrolysed crude casein reduced the cholesterol level in orthophthaldialdehyde (OPA) assays by 39.5%, while the 10 kDa and 1 kDa permeates of casein hydrolysed with trypsin for 4 h reduced the cholesterol level by 50.7 and 69.6%, respectively. The unhydrolysed crude casein and the 10 kDa and 1 kDa permeates of casein hydrolysed by trypsin for 4 h inhibited ACE activity by 18.9%, 29.7%, and 51.4%, respectively.
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