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Background: Estimation of glucose is very important in clinical diagnosis of diabetes mellitus, hyperglycemia and hypoglycemia. The core aim of clinical laboratory service is to produce accurate, precise and reliable test results. The ability of laboratories to do this lies in the availability of well trained personnel, equipment, electricity etc. There has been a progressive increase in the prevalence of diabetes mellitus in Nigeria and the burden is expected to increase even further and the need for proper diagnosis cannot be overemphasized. Aim: In this study we have tried to evaluate the impact of delayed separation of plasma and serum from cells and clot on glucose level. Method: A total of fifty (50) randomly selected apparently healthy students of Federal School of Medical Laboratory Technology (Science), Jos were recruited as subject into this study. Blood samples were collected from each of the subjects into plain and anticoagulant bottles for serum and plasma respectively. An aliquot was analyzed within 10 minutes to obtain a baseline value where other values after timely delays were compared. The samples were spun but not separated from the cells/clot all through the period of the analysis. Blood glucose was determined by glucose Oxidase Colourimetric assay kit obtained from Randox Laboratories Limited United Kingdom. Data were analyzed using student’s t-test and performed using the Statistical Package for Social Sciences (SPSS) version 20.0. Results: Result obtained shows the mean and standard deviation of all timing for plasma and serum. In plasma it shows that the means±SD of 2, 4 and 24hours (5.2±0.7, 5.1±0.4 and 5.3±0.5 respectively) were not significantly varied when compared to the baseline (0 hour) value (5.3±0.5). That of baseline and 24 hours (5.3±0.5 and5.3±0.5 respectively) were significantly higher (P<0.001) than that of 48 and 74 hours (1.0±0.20 and 7±0.2 respectively). In serum, similar results were obtained. Conclusion: In conclusion, where possible, it is advised that the established best practices in processing and analysis of samples be adhered to. However, the results of this work shows reasonably stable results for blood glucose determination could be obtained within 24 hours. Therefore, instead of discarding the sample, it might seem more appropriate that the sample be analyzed and the result reported indicating the number of hours delayed within 24hours.

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This study was carried out between March 2014 and June 2014 on the occurrence of malaria infection in under five children in Orlu Local Government area of Imo State Nigeria. The study covered 381 randomly selected households and 622 consenting subjects with information's gotten mostly from mothers and nannies as care providers. The study population was 510 children below 5 years of age. Analysis of data collected shows that 77% of respondents in the rural area had no education above secondary school level, while 56% of respondents in the sub-urban area had education up to secondary school and 67% of respondents in the urban area had education up to the secondary school level this incidence of malaria in the studied areas in inversely proportional to the socio-economic levels of the areas in under study Incidence increased with decrease in socio-economic levels and the improvement in living standard. Use of bed nets is more common in communities with better socio economic status. Regarding management of Malaria ie purchase of across-the counter drugs use of leftover drugs, application of traditional medication etc. was highest in Amike and decrease to the lowest in the urban local Government headquarters. Also malaria related complications were more among the children from low socio-economic households than their counterparts of high socio-economic level.