The proximate, functional, antinutrient and antimicrobial properties of avocado pear (Persea americana) seeds were studied using standard methods. The proximate contents (%) of avocado peat seed flour, ASF, in decreasing order were moisture (13.09±0.14) followed by ash (3.82±0.00), fibre (2.87±0.00), protein (2.64±0.01) and fat (0.33±0.00). The energy value, carbohydrate and dry matter content respectively was 334.01±1.05%, 80.12±0.15% and 86.91±0.14% while the water absorption capacity and oil absorption capacity respectively was 2.70±0.00% and 1.80±0.00%. The anti-nutrients (mg/100g) in decreasing order were saponins (8.10±0.01) followed by alkaloids (2.14±0.00), flavonoids (1.81±0.01), tannins (1.14±0.01), cyanogenic glycosides (1.02±0.00) and phenols (0.29±0.01). The avocado pear seed extract (ASE) elicited antibacterial activity (mm) against Proteus mirabilis (23±0.14), Staphylococcus aureus (16±0.04) and Pseudomonas aeruginosa (15±0.11) though lower than the corresponding activity by the standard, Ciprofloxacin. ASE had higher activity (18±0.31mm) against the fungus, Aspergilus niger compared with the standard antifungal, Ketoconazole (8±0.22mm) while it had a comparable activity as the standard against Candida albicans but no activity against Penicillum notatum in contrast to the standard drug (6±0.24mm). Thus, ASF had appreciable nutrient and antinutrient contents with apparently moderate storage value while, out of the tested pathogens, ASE had a broad spectrum antibacterial activity, a selective antifungal activity and an overriding activity against Aspergilus niger. These while highlighting possible diet and drug potentials of ASF and ASE, respectively, provided basis for ethno-medicinal applications of avocado pear seeds, hence warranting further studies.