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Vitamin A deficiency (VAD) is prevalent in developing countries. Socioeconomic status is increasingly being associated with nutritional status of individuals. Efforts to control or eliminate VAD must focus on socioeconomic factors and how to tackle them in the midst of other well recognized strategies. This research sought to determine the socioeconomic factors that may affect the serum retinol levels of pregnant women in Calabar, Nigeria. A total of 101 pregnant women in Calabar were studied for the effect of socioeconomic factors on the maternal vitamin A status using a structured household questionnaire and biochemical analytical techniques. The study revealed that a majority (82.2%) of the women studied were in their normal reproductive years (20-45 years), 63.4% had previous childbirth experience, 89.1% had formal education, 85% were employed, 85% lived in medium to large families (4 persons and above) while 62.3% were in the middle or high income class (over N50,000 per month). Among the women, only 35.6% had adequate vitamin A status while the rest had deficient (15.8%), low (32.7%) or very high (15.8%) levels. The results of the analysis of the influence of socioeconomic characteristics of the women on serum retinol status showed a positive relationship between serum retinol level and education (P < 0.05; F = 2.84) and between serum retinol level and parity (P < 0.05; F = 2.05). Serum retinol concentration was not affected by age, occupation, household size and other household characteristics used in the study. Increased maternal education and appropriate child spacing may be the key strategies for the elimination and control of vitamin A deficiency in developing countries.

Aim: Eggs have sometimes been regarded as unhealthy foods due to their relatively high cholesterol content. The aim of this study is to determine contribution of eggs and other    cholesterol containing food to total dietary cholesterol and their influence on serum lipid profile of adults. Study Design: Cross sectional and experimental. Place and Duration of Study: Department of Biochemistry, University of Calabar, Calabar. February to July 2017. Methodology: A cross-sectional survey to determine consumption pattern was carried out on 400 respondents using food frequency questionnaire (FFQ) and 24 hour dietary recall. The respondents were further grouped into four based on their reported weekly egg consumption. A detailed follow-up study was conducted on 50 participants selected from across the 4 groups, using a 3-day repeated 24 hour dietary recall to determine their consumption of egg and other cholesterol-containing foods. Serum blood lipid profile of these 50 participants was also determined using Randox cholesterol test kits. Food composition tables were used to calculate dietary cholesterol intake (DCI). The mean DCI of the 4 groups were cross-tabulated with mean serum cholesterol levels. Percentage contribution of eggs and other frequently consumed cholesterol-containing foods (such as milk and fish) to total DCI was calculated. Statistical significance was accepted at p = .05. Results: For the follow-up participants, it was observed that results of correlations between DCI and the lipid profile parameters showed negative correlation (at p = .01) in both males and females, except slight positive correlations between cholesterol intake and HDL-c (r=0.191) among the males, and cholesterol with TC (r=0.265) among the females. Apart from this, no association was observed between DCI and the lipid profile parameters. Furthermore, the > 5eggs/week group had the lowest TC and LDL-c (4.23±0.19 mmol/L and 2.38±0.10 mmol/L). Based on the respondents’ consumption patterns, eggs (boiled and fried) contributed the highest- 34.8% to total DCI, followed by milk (15.9%); salad cream contributed lowest (0.3%) to total DCI. Conclusion: Increased DCI from cholesterol-containing foods (such as eggs), did not cause an adverse increase in serum cholesterol levels of normocholesterolemic people.

Aflatoxicosis is a public health problem in Nigeria like other tropical and sub-tropical regions of the world. Control of aflatoxin contamination requires thorough risk assessment, monitoring, quality control and empirical data. This study assayed total aflatoxin levels, identified and quantified four aflatoxin types in five food commodities commonly consumed in the six geopolitical zones of Nigeria. The food materials: <em>Zea mays, Colocynthis citrullus, Capsicum frutescens, Irvingia gabonensis</em> and <em>Arachis hypogea</em> were obtained from Watt market in Calabar urban. ELISA method was used for total aflatoxin, HPLC for aflatoxin types, AOAC for moisture. All (100%) the samples were contaminated with aflatoxin. Contamination was highest in<em> Irvingia gabonensis</em> (63.40 ± 1.79 µg/kg) and least in <em>Zea mays</em> (3.20 ± 0.12 µg/kg) (p &lt; 0.05). Except for <em>Irvingia gabonensis</em> and <em>Colocynthis citrullus</em>, total aflatoxin was within safe intake level of the Nigerian regulatory authority (National Agency for Food and Drug Adminstration and Control {NAFDAC}). All four aflatoxin types occurred in <em>Irvingia gabonensis</em>, <em>Capsicum frutescens</em> and <em>Colocynthis citrullus</em>; none was detected in <em>Arachis hypogea</em>. AFB₁ contamination was highest in<em> Irvingia gabonensis</em> (11.71±0.10 µg/kg) followed by <em>Capsicum frutescens</em> (1.21 ± 0.01 µg/kg); AFB₂ ranged from 0.00 ± 0.00-2.43 ± 0.05 µg/kg, AFG₁0.00 ± 0.00-3.73 ± 0.04 µg/kg, and AFG₂ 0.00 ± 0.00-0.54 ± 0.01 µg/kg (p &lt; 0.05). Only<em> Irvingia gabonensis</em> exceeded the limit of AFB₁ specified by NAFDAC for human foods. Moisture content varied widely (3.23 ± 0.03%-10.37 ± 0.19%).<strong> </strong>The trend in the occurrence of aflatoxins in the food samples was directly proportional (r = 0.91) to their moisture contents. Food<strong> </strong>commodities sold in Calabar carry potential health hazard. Improved handling through food processing, preservation and storage can minimize aflatoxins in foodstuffs and ensure sustainable quality of food supply.

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The proximate and elemental compositions of various body parts of Macrobrachium macrobranchion (prawn) obtained from the Great Kwa River, a major tributary of Cross River estuary in Cross River State, Nigeria were investigated using standard methods of AOAC. Results showed that the flesh had significantly higher (p < 0.05) levels of protein, fat and moisture (22.32, 7.70 and 58.40%, respectively) than the other body parts analyzed. Equally high in protein were the head (20.11%) and appendages (19.28%), while the exoskeleton recorded the least protein content (14.02%). The flesh had the least (p < 0.05) crude fibre (0.03%) and carbohydrate (7.22%) contents, and conversely had the least energy value (187.50 kcal/g) among the body parts. Ash content was significantly higher (p < 0.05) in the exoskeleton (7.14%), the appendages (7.01%) and the head (6.05%) than in the flesh (4.30%). Individual elements were also unequally distributed among the four body parts investigated: sodium and potassium were more concentrated in the flesh (189.27 mg/100 g and 114.70 mg/100 g, respectively), while calcium and magnesium were highest in the appendages (99.02 mg/100 g and 171.40 mg/100 g, respectively). The concentration of iron was generally low among the body parts; however, it was highest (p < 0.05) in the head. The usual practice of retaining the flesh and discarding the "hard" parts (head, exoskeleton and appendages) of prawn during food preparation should be discouraged as this may promote wastage of important nutrients.

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