И. В. Булавин, нс лаб. геномики растений и биоинформатики, к.б.н. 1 В. А. Браилко, зав. лаб. геномики растений и биоинформатики, к.б.н. 1 О. А. Гребенникова, снс лаб. геномики растений и биоинформатики, к.б.н.1 М. С. Андреев, мнс лаб. геномики растений и биоинформатики 1,2 О. В. Кривенко, внс лаб. геномики растений и биоинформатики, к.б.н.1 И. В. Митрофанова, зав. отделом биологии развития растений, биотехнологии и биобезопасности, д.б.н.
In production, shoot cuttings of lavender are most often used for vegetative propagation, however, this method does not promote high propagation rate. The most effective method is propagation by axillary or terminal buds that requires further plant rooting in vitro. Due to, objective of our work was analysis of lavender in vitro rhizogenesis. Investigation was performed on Lavandula angustifolia cultivars with different concentration of the growth regulators in medium. After18 Day, Only 1 mg/l NAA did not stimulate rhizogenesis for ‘Record’ and ‘Sineva’ cultivars. Root primordia formed from the cambium cells. Further root growth accompanied by cleavage of the core tissues and they appeared on the shoot surface. Morphologically, a root cap, meristem, elongation zone and zone with root hairs were identified in de novo formed root in vitro. Along with normally roots, the appearance of roots accreted along their periphery with free apexes and roots with the decreased meristem was also noted. For ‘Prima’ cultivar, highest values of the mitotic index were observed on the hormone-free half-strength MS medium and on ½MS medium with 1.0 mg/l NAA. Thus, our data showed that root morphogenesis for lavender cultivars depended on the plant material and culture medium.
Common hyssop (Hyssopus officinalis L.) is widely used as an aromatic and medicinal plant. In the component composition of the essential oil from the above-ground mass of H. officinalis f. cyaneus, from the collection of the Nikita Botanical Garden, the bicyclic monoterpene ketones isopinocamphone (50.99–64.41%) and pinocamphone (3.95–18.88%) predominate, which allows us to attribute this form to the isopinocamphone chemotype for use in pharmacology. An essential oil sample with a high content of isopinocamphone (70.74%) in complete absence of pinocamphone was determined, which made it possible to use the plant as a starting material for breeding. The objective of our work was to study the component composition of the essential oil of this form, and the morphology, anatomy and ploidy level of microshoots in vitro on a nutrient medium with BAP. This was compared with ex situ samples to develop a cultivation technique with the preservation of a valuable trait for use in task-oriented selection. Biotechnological methods are used for future mass propagation, study and the preservation of breeding forms. Morphological and anatomical features and ploidy of H. officinalis microshoots were studied in vitro. Using in vitro culturing of microshoots on MS medium containing BAP, a decrease in the cuticular layer and the degree of development of collenchyma near vascular bundles in leaves were shown compared to microshoots ex situ. Significant structural changes were found with a high increase in BAP concentration, while no changes in the ploidy level were detected.
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