Mangiferin, a bioactive metabolite having potent antioxidant and pharmacological properties has been obtained in this work by microwave assisted extraction (MAE) technique, from Mangifera indica leaves. A BoxBehnken design was used to evaluate the effect of three parameters: microwave power, the time of extraction, and the ratio of solvent to raw material (mL/g) over the mangiferin yield. Irradiation time was the most important parameter on the recovery of mangiferin. The results indicated that the optimal conditions should be as follows: power of microwave 900 W, extraction time of 5 min and a solvent volume / vegetal material relation of 10. With these parameters the maximum mangiferin yield was 63.22%. The MAE process was compared with the conventional extraction in stirred tank. No significant statistical differences were found (p= 0.5639) between both extraction methods of mangiferin. The presence of the metabolite was confirmed using high performance liquid chromatography. The method was validated with the guidelines set on the International Conference on Harmonization (ICH), for the validation of analytical procedures.
-Mangiferin is a natural xantone extensively distributed in higher plants showing different pharmacological properties. In the present study, an ultrasonicassisted extraction (UAE) method was evaluated for obtain mangiferin from Mangifera indica L. (c.v Mango Macho) leaves. Differents extraction parameters were optimized using a response surface methodology and the best conditions reached were at 47% ethanol concentration, a liquid-to-solid ratio 47:1, and an extraction time of 63min. under ultrasound irradiation of 150W. In these circumstances, the yield of mangiferin was 24.15±0.015mg/g. For first time, was evaluated the ultrasound assited extraction to higher frecuency and the value of the yield reached was 6.38± 0.062mg/g. Comparison of UAE of mangiferin respect to microwave and aggited tank was realized and the yield obtained by UAE was inferior, however the ultrasonic time was minor respect to conventional extraction technique. The ultrasonic-assisted extraction is an alternative method to use for the extraction of mangiferin from Cuban mangos.
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