All maxillary first premolars subjected to RME showed ERR and partial cementum repair. Banded teeth did not develop more ERR than nonbanded anchorage teeth.

Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. Gingival fibroblasts present structural function, being able to modulate their metabolic capacity, which is reflected in the tissue morphology. The possibility of culturing fibroblasts in vitro, in monolayer or on three-dimensional scaffolds, for subsequent transplants in vivo opens important perspectives for the periodontal surgical clinic. The objective of the present article is to present a method of obtaining and cultivating viable human gingival fibroblasts for in vitro research. Explants derived from periodontal surgical discards were used, grown in 25 cm 2 bottles to obtain a primary cell culture. After observing the proliferation and growth of the fibroblasts that interconnected and formed a monolayer network, involving the periphery of the explants, it was possible to remove the explants, to make the passage and the new subcultures were obtained in a ratio of 1:1. After 7 days, the amount of viable cells was analyzed in triplicate, using the Neubauer chamber technique, in cell culture bottles of 25 mm 2 (T25) and 75 mm 2 (T75). Fibroblasts were described and subclassified morphologically. The results showed a growth pattern in both bottles, but with a larger number in bottles of 75 cm 2. Cells with fibroblastic morphology were subclassified into reticular and fusiform, being predominant those with fusiform morphology. In conclusion, culture of explant of human gingival connective tissue is a viable method for obtaining gingival connective tissue cells suitable for laboratory tests in cell culture, aiming at obtaining constructs for gingival tissue engineering.

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Background : Cell culture is an important tool in medical and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. Gingival fibroblasts present structural function, being able to modulate their metabolic capacity, which is reflected in the tissue morphology. The possibility of culturing human fibroblasts in vitro, in monolayer or on three-dimensional scaffolds, for subsequent transplants in vivo opens important perspectives for the periodontal regeneration.Aim/Hypothesis : The objective is present a method to obtaining and culture of the viable human gingival fibroblasts for in vitro research Material and Methods : Explants derived from periodontal surgical discards were used, grown in 25 cm2 bottles to obtain a primary cell culture.After observing the proliferation and growth of the fibroblasts that interconnected and formed a monolayer network, involving the periphery of the explants, it was possible to remove the explants, to make the passage and the new subcultures were obtained in a ratio of 1-1. After 7 days, the amount of viable cells was analyzed in triplicate, using the Neubauer chamber technique, in cell culture bottles of 25 mm2 (T25) and 75 mm2 (T75). Fibroblasts were described and sub classified morphologically.Results : The results showed a growth pattern in both bottles, but with a larger number in bottles of 75 cm2. Cells with fibroblastic morphology were sub classified into reticular and fusiform, being predominant those with fusiform morphology. Conclusion and ClinicalImplications : Culture of the explant of human gingival connective tissue is a viable method for obtaining gingival connective tissue cells suitable for laboratory tests in cell culture, aiming at obtaining constructs for gingival tissue engineering.

Background : Fresh and frozen allogeneic bone grafts, from Bone Bank Allograft, are configured as alternative resources to biomaterials and autogenous grafts.Aim/Hypothesis : The present study evaluated histologically five samples of fresh, treated and frozen human bone tissue obtained from the UNIOSS® bone bank (Brazil, Campinas-SP).Material and Methods : The cortico-medullary samples, from different donors, were legally obtained by an accredited professional and qualified in Implantology. The fragments were removed prior to the surgical procedure of reconstructive bone grafting. The samples were fixed in 10% formalin, demineralized in 10% EDTA, paraffin embedded and stained in HE, Masson and Gomori trichrome.Results : Microscopy revealed a characteristic trabecular aspect. Cell remains as well as intercellular matrix residues were observed in the medullary spaces. In some samples cartilaginous tissue and chondrocyte remains were observed. Conclusion and Clinical Implications :We concluded that the morphology of the collagen matrix of the bones from bone bank allograft may contribute to cell adhesion for bone repair. However, the eventual presence of hyaline cartilage and cell resources, in these bone fragments, may influence the repair process. These findings justify, at least partially, the controversy regarding the clinical results and the lack of consensus of the professionals regarding this type of therapy for bone reconstruction.