Objective Rice husk nanosilica has a porous, amorphous structure with a silica (SiO2) surface. Silica interacts with calcium ions to form hydroxyapatite and can induce the formation of reactive oxygen species (ROS), which harm microorganisms. This research determines the effect of rice husk nanosilica on the increase in dentin hydroxyapatite and its antimicrobial effects against Streptococcus mutans.
Materials and Methods We divided 27 dental cavity samples into three groups (n = 9). Group 1: normal dentin, Group 2: demineralized dentin, Group 3: demineralized dentin treated with rice husk nanosilica. The samples were analyzed using X-ray diffraction (XRD) to evaluate the formation of dentin hydroxyapatite. To analyze the viability of S. mutans after exposure to 2% nanosilica rice husk, we conducted an antimicrobial MTT assay.
Statistical Analysis The Kruskal–Wallis test evaluates the formation of dentin hydroxyapatite, and the t-test evaluates the viability of S. mutans.
Results There was an increase in the amount of dentin hydroxyapatite after the application of rice husk nanosilica compared with the control group (normal dentin), and 2% rice husk nanosilica had an antimicrobial effect (p < 0.005) in the group exposed to it.
Conclusion Rice husk nanosilica can induce the formation of dentin hydroxyapatite and has antimicrobial effects.
Mineral trioxide aggregate (MTA) diketahui memanfaatkan sifat reaktif gugus hidroksil dari permukaan nano silika yang dikandungnya untuk remineralisasi jaringan. Sementara itu, silika berukuran nano dapat diekstraksi dari sekam padi melalui metode sol-gel dan pirolisis. Tujuan penelitian menganalisis perbedaan intensitas transmitansi pelepasan senyawa hidroksil yang dihasilkan oleh MTA, nano silika sekam padi hasil metode sol-gel dan pirolisis. Metode: Jenis penelitian eksperimen laboratori. Sebanyak 3 sampel yang terdiri dari masing-masing bubuk sampel MTA, nano silika sekam padi sol-gel dan nano silika sekam padi pirolisis sebanyak 0,14 mg dicampurkan dengan 0,2 ml aquades dan dibiarkan mengeras selama 15 menit. Hasil campuran kemudian diukur menggunakan alat Fourier Transform Infrared Spectroscopy (FTIR) dan dilihat intensitas transmitansinya. Hasil karakteristik FTIR kemudian dianalisis secara kualitatif. Hasil: Ketiga sampel uji melepaskan gugus fungsi -OH di kisaran gelombang 3000-3500. Sampel MTA memperlihatkan intensitas puncak transmitansi gugus -OH paling tinggi. Sample nano silika sol-gel memperlihatkan intensitas puncak transmitansi gugus -OH lebih tinggi dibandingkan nano silika pirolisis. Sampel MTA memperlihatkan keberadaan gugus karbonat (CO 3 -2 ) yang tidak terdapat pada kedua sampel nano silika, sementara kedua sampel nano silika memperlihatkan gugus siloksan (Si-O-Si) yang tidak terdapat pada sampel MTA. Simpulan: MTA menghasilkan intesitas transmitans senyawa hidroksil paling tinggi, yang menandakan MTA dapat melepaskan gugus hidroksil paling banyak dibandingkan sampel nano silika sol-gel dan nano silika pirolisis. Kata Kunci: Mineral Trioxide Aggregate (MTA), nano silika sekam padi metode sol-gel, nano silika sekam padi metode pirolisis, pelepasan senyawa hidroksil, Fourier Transform Infrared Spectroscopy (FTIR).
Differences in the transmittance intensity of hydroxyl release from mineral trioxide aggregate, sol-gel, and pyrolysis nano-silica of rice huskABSTRACT Introduction: Mineral trioxide aggregate (MTA) is known to take advantage of the reactive properties of the hydroxyl groups from the surface of the nano-silica for tissue remineralisation. Meanwhile, nano-sized silica can be extracted from rice husks through sol-gel and pyrolysis methods. The research objective was to analyse the differences in the transmittance intensity of hydroxyl compounds released by MTA, nano-silica of rice husks resulting from the sol-gel method and pyrolysis. Methods: This research was an experimental laboratory. A total of 3 samples consisted of MTA powder, sol-gel nano-silica rice husk and pyrolysis nano-silica rice husk, 0.14 mg each, mixed with 0.2 ml of distilled water and hardened still for 15 minutes. The mixture results was then measured using a Fourier Transform Infrared Spectroscopy (FTIR) instrument, and then the intensity of the transmittance was observed. The results of the FTIR characteristics were then analysed qualitatively. Results: All samples release the -OH functional group in the wave ran...
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