Moringa oleifera Lam. (MO) is a small size multipurpose tree of approximately 5 to 10 m in height which is cultivated all over the world. The study was conducted in the Guinea Savanna and the semideciduous forest zones of Ghana in December 2011 to compare the nutrient levels of MO leaves from the two agro-ecological zones. Leaf samples were collected from three randomly selected districts in each ecological zone for proximate and chemical analysis of some macro and micro nutrient. The results show no significant difference in crude protein and carbohydrate levels of MO leaves from the two ecological zones, however MO leaves from the semi-deciduous forest zone recorded a slightly higher mean crude protein value of 26.54% than the Guinea savanna value of 25.65%. MO leaves from the semi-deciduous forest and the Guinea Savanna recorded mean calcium values of 1880 and 1474.33 mg per 100 g of leaf powder, respectively. The comparison of mean values of potassium and iron of MO leaves showed no significant difference between MO leaves from the two ecological zones. The research concluded that agro ecological zonation had no significant effect on the levels of most nutrients in MO leaves.