Eugenol has many functional properties for food and pharmaceutical purposes, especially as an antimicrobial agent. However, its use is constrained by its volatility and shelf life because it is easily degraded due to temperature, oxidation, and light. Research on encapsulation technology using biopolymers is still required to obtain the appropriate formulation in a eugenol delivery system. The aims of this research were to develop a new formulation of protein and polysaccharides in eugenol encapsulation and to determine the effect of eugenol and chitosan concentration on the characteristics of the emulsions and spray-dried powder produced. In this study, eugenol was encapsulated in whey protein–maltodextrin conjugates and chitosan through the double layer encapsulation method. The emulsions which were prepared with 2.0% eugenol were relatively more stable than those of 1.0% eugenol based on the polydispersity index and zeta potential values. Spray-dried powder which was prepared using an emulsion of 2.0% w/w eugenol and 0.33% w/w chitosan had the highest eugenol loading. The presence of chitosan resulted in more stable emulsions based on their zeta potential values, improved thermal stability of eugenol, increased eugenol loading to become twice as much as the loading obtained without chitosan, and modified release profile of eugenol from the spray-dried powders.
<p>Minyak biji pala mengandung senyawa bioaktif yang memiliki aktivitas antimikroba. Senyawa bioaktif umumnya bersifat tidak stabil sehingga mudah mengalami penurunan aktivitas biologisnya. Salah satu cara untuk meningkatkan stabilitasnya adalah dengan menggunakan teknologi nano. Penelitian ini bertujuan untuk (1) mendapatkan formulasi nanoemulsi minyak biji pala dengan ukuran droplet partikel <300 nm sebagai bahan antimikroba, (2) mendapatkan formulasi nanoemulsi yang memiliki aktivitas antimikroba terbaik terhadap mikroba E.coli, S.aureus dan S.cereviseae. Proses nanoemulsi minyak biji pala dilakukan dengan menggunakan alat High Pressure Homogenizer pada tekanan 20000 psi atau 137,931 KPa dengan 5 siklus. Pengamatan dilakukan terhadap 12 formulasi yang diperoleh dari kombinasi antara tiga konsentrasi minyak (5%, 10% dan 15%) dan dua jenis surfaktan (tween 20 dan tween 80) dengan tiga tingkat konsentrasi (10%, 15% dan 20% dari massa minyak biji pala). Pengujian aktivitas antikmikroba dilakukan dengan metode difusi sumur. Hasil penelitian menunjukkan bahwa formulasi nanoemulsi minyak biji pala yang diperoleh memiliki ukuran droplet partikel 104,80-161,15 nm.. Konsentrasi minyak biji pala berpengaruh secara signifikan terhadap zona penghambatan pertumbuhan mikroba. Nanoemulsi minyak biji pala dengan formulasi konsentrasi minyak biji pala 15% dan jenis surfaktan Tween 80 dengan konsentrasi 20% dari massa minyak (M15S20T80) memberikan efek penghambatan terbaik pada E.coli (11,25 mm), S.aureus (13,06 mm) dan S.cereviseae (11,4 mm).</p><p>Kata kunci :minyak biji pala, surfaktan, nanoemulsi, antimikroba, penghambatan</p><p>English Version Abstract</p><p>Nutmeg oil contained bioactive compounds which had antimicrobial activities. Generally, bioactive compounds have a limited stability, so that they undergo biological activity degradation easily. One of ways to increase its stability is using nanotechnology. This research was aimed (1) to obtain nutmeg oil nanoemulsion formulation which having particle size <300 nm as antimicrobial agent, (2) to obtain nanoemulsion formulation which having the best antimicrobial activity toward E.coli, S.aureus and S.cereviseae. Processing of nanoemulsion was conducted using High Pressure Homogenizer at 20000 psi or 137,931 Kpa and 5 cycles. Observation was conducted to 12 nanoemulsion formulation which was gained from combination between concentration of nutmeg oil (5%, 10%, 15%) and types of surfactant (tween 20 and tween 80) with 3 level concentration (10%, 15% and 20% from nutmeg oil mass). Antimicrobial testing was conducted using agar well diffusion method. The result showed that formulation of nutmeg oil nanoemulsion having particle size 104,80-161,15 nm. Nutmeg oil concentration had significantly effect in inhibition zone of microbes growth. Formulation of nutmeg oil nanoemulsion with 15% nutmeg oil and surfactant tween 80 as much as 20% of nutmeg oil mass gave the best growth inhibition on E.coli (11,25 mm), S.aureus (13,06 mm) dan S.cereviseae (11,4 mm).</p><p>Keywords :nutmeg oil, nanoemulsion, antimicrobial, inhibition</p>
Clove oil has benefits for both food and pharmaceutical purposes. However its use is limited due to several properties such as susceptibility to oxidation, poor water solubility, react with other components, and toxic at high concentration. The limitation of its usage can be overcome through emulsion technology. Therefore, this study aims to obtain clove oil nanoemulsion through preparation using whey protein-maltodextrin conjugates. These conjugates act as an emulsifier and a first layer, meanwhile chitosan as a stabilizer and also a second layer. The formulation used different chitosan concentrations, at 0.2, 0.4, and 0.6%, then processed using High-Pressure Homogenizer (HPH) in 3 and 5 cycles. The result showed that the concentration of chitosan and the number of HPH cycles affected the clove oil emulsion performance. Furthermore, the formulation using 0.6% chitosan concentration and 5 cycles of HPH produced the best clove oil nanoemulsion in droplet size, polydispersity index, and zeta potential were 147.5, 0.214, and -52.4, respectively. The results of this study provide information on the potential of emulsion formulations using WPM conjugate and chitosan to produce clove oil nanoemulsions that can be utilized in food and pharmaceutical products.
There is a lack of information regarding the characteristics and potential of specialty corn in Indonesia, resulting in its limited use as a raw material in the corn processing industry. Starches were isolated from four varieties of biodiversity-specialty corn (Srikandi Kuning, Srikandi Ungu, Pulut Uri, and Provitamin A), one line of specialty corn (Dent-type), and one imported corn from Argentina. The aim of this study was to gather data on the physicochemical and functional properties of Indonesian specialty corn starch, which is to be used as the base to increase the value of those biodiversity. Starch yields ranged from 34.30 to 49.22%. The starches met the SNI (Indonesian National Standard) requirements for both water content (6.90–8.72%) and white degree value (86.40–105.46%). There were variations in the starch contents of starches from 72.03 to 82.75%, and in the amylose contents from 6.32 to 31.47%. Starch granules showed oval and polygonal shapes with sizes ranging from 2.93 to 18.36 mm. Pasting profile and thermal properties showed the differences among the starches. The Dent-type corn starch had the lowest breakdown value of all the Indonesian specialty corn types, while imported corn starch had the lowest breakdown value of all the starches, showing the greatest stability during heating and stirring. In terms of peak temperature and enthalpy value, they ranged from 82.9°C to 92.71°C and from 85.94 to 551.93 J/g. The present study is expected to provide information about characteristics of Indonesian specialty corn starch, then subsequently, it can be utilized by the food industry, and increase the value of our national biodiversity.
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