Breast cancer is the most common type of tumor in women worldwide. Immune checkpoint inhibitors, particularly anti-PDL1, have shown promise as a therapeutic approach for managing this disease. However, this type of immunotherapy still fails to work for some patients, leading researchers to explore alternative immune checkpoint targets. The Ig suppressor of T cell activation domain V (VISTA) has emerged as a novel immune checkpoint that delivers inhibitory signals to T cells and has demonstrated encouraging results in various cancers. Our study investigated the association of VISTA expression with clinicopathological parameters in breast cancer patients, its involvement in the Epithelial-Mesenchymal-Transition (EMT) process, and its correlation with PD1 expression. Transcriptomic analysis revealed that VISTA was associated with lobular and metaplastic histological type, tumor size, lymph node status, ER and PR negative status, and the TNBC molecular subtype. Furthermore, VISTA expression was strongly associated with an immunosuppressive tumor microenvironment. Immunohistochemistry analysis corroborated the transcriptomic results, indicating that VISTA was expressed in most immune cells (94%) and was significantly expressed in breast cancer tumor cells compared to matched adjacent tissues. Our study also showed for the first time that VISTA overexpression in breast cancer cells could be associated with the EMT process. Additionally, we identified a positive correlation between VISTA and PD-1 expression. Together, these results highlight the immunosuppressive effect of VISTA in breast cancer patients and suggest that bi-specific targeting of VISTA and PD-1 in combination therapy could be beneficial for these patients.
The Sidi Ifni region in southwest Morocco is mainly composed of crystalline rocks with limited groundwater storage capacity. These water resources drain in particular fault zones with high fracture permeability. The main objective of this study is to describe the geological structure of the region to optimize future drilling locations. The gravity data were processed using various techniques, such as total horizontal gradient, tilt derivative, and Euler deconvolution, in conjunction with the interpretation of the geological data, to create a new structural map. This map confirms the presence of many previously identified or inferred faults and identifies significant new faults with their respective trends and depths. Analysis of this map shows that major faults are oriented NNE-SSW and NE-SW, while minor faults are oriented E-W, NW-SE, and NNW-SSE. The superposition of the hydrogeological data and the structural map reveals that the high groundwater flow values in the boreholes are located in the vicinity of the major faults and talwegs. The structures deduced from the filtering and interpretation of the gravity data suggest that the hydrogeological system of the Ifni Inlier is controlled by its structures. To confirm this impact, a high-resolution electrical resistivity map (7200 Hz) was used, with penetration depths ranging from 84 to 187 m. Negative boreholes, located in high resistivity ranges corresponding to sound basement formations without fault crossings, showed high resistivity values. The positive holes, located in anomalies with low linear resistivity, revealed the impact of fault crossings, which drain water and tend to decrease the resistivity values of the formations. Therefore, these new structural maps will assist in planning future hydrogeological studies in this area.
Bronchogenic cysts are rare unusual congenital cysts, resulting from abnormal budding of the primary tracheobronchial tube and can be located either in the mediastinum or in the pulmonary parenchyma. They remain asymptomatic in most adults unless they are large to compress adjacent structures or infected. We report a case of a 43-year-old woman, who presented recurrent episodes of hemoptysis low abundance 4 months ago revealing a huge mediastinal cystic formation, initially taken as a hydatid cyst, the chest CT showed a voluminous cystic formation of 11,4x10, 9x8, 8 cm exerting a mass effect on the posterior mediastinal structures: the superior vena cava, the azygos vein, and the trachea. The diagnosis as a bronchogenic cyst is done after resected by uniportal video-assisted surgery (VATS), and histological confirmation. The patient does not present any complications or reoffending during the follow-up. The rarity of this entity and even more a rare association of this lesion with symptoms of hemoptysis led to the report of this case. This case illustrates the interest of uniportal VATS for the extirpation of the bronchogenic cyst. We recommend a complete exeresis in most cases to confirm the diagnosis, relieve symptoms, and prevent complications.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.