The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding. The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH. The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed.
Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (7l), 2 (2l), 4 (4l), and 5 (1l) by a two-step in situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have designated the new locus Nor-l6. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in other Triticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.
The paper reviews recent literature on the cytology, cytogenetics, molecular cytogenetics, and gene structure of barley, and on the relationship of the barley and wheat genomes. The information collected demonstrates largely similar genetic contents and collinearity of gene loci in the seven chromosomes of barley and in those of other members of the Triticeae. The high level of synteny indicates that the whole group can be considered as a single gene pool. On this basis, the 7th International Barley Genetics Symposium recommended that (i) the currently used designations of the barley chromosomes (the BURNHAM and HAGBERG system) should become secondary to the use of the Triticeae system; i.e., each of the seven barley chromosomes is designated by a numeral between 1 and 7 according to its homoeologous relationship with the chromosomes of the wheat genomes followed by the genomic symbol H, e.g., 2H; (ii) the genomes of Hordeum vulgare and H. bulbosum are symbolized with the letter H; and (iii) the arms of the barley chromosomes are designated by the capital letters S and L (cf. SINGHand TSUCHIYA 1982b). Further, it was recommended that the barley genome present in the barley cultivar ‘Betzes’ should become the reference genome in the Triticeae.
Watercress obtained in food stores in the United States contained significant levels of epiglucobarbarin [(R)-2-hydroxy-2-phenylethylglucosinolate] and low levels of the 2S-epimer glucobarbarin identified by an HPLC+NMR+MS/MS approach. Typical combined levels were 4-7 μmol/g dry wt. The hydrolysis product, 5-phenyloxazolidine-2-thione (barbarin), was detected at similar levels as the precursor glucosinolates after autolysis of fresh watercress in water. Fragmentation patterns in MS(2) of reference desulfoglucosinolates were side chain specific and suitable for routine identification. Watercress was of two main glucosinolate chemotypes: Material from U.S. food stores had a complex profile including glucobarbarins, gluconasturtiin, indole glucosinolates and high levels (6-28 μmol/g dry wt.) of long-chain methylsulfinylalkyl and methylthioalkyl glucosinolates. Material from European food stores had a simple profile dominated by gluconasturtiin, with low levels of epiglucobarbarin and moderate levels of indole glucosinolates. Some wild U.S. material was similar to the U.S. food store type. Both types were found to be Nasturtium officinale by floral parts morphology. Cytological analysis of one U.S. food store accession indicated that it represented a chromosome-doubled variant within N. officinale. The nutritional consequences and invasive potential of the U.S. food store chemotype are discussed.
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