Forage alfalfa (Medicago sativa L. [Fabales: Fabaceae]) is a key agricultural commodity of the western region of the United States. The key insect pest of alfalfa, Hypera postica Gyllenhal (Coleoptera: Curculionidae), has developed resistance to the most common class of insecticide used to manage its damage. Alfalfa weevil samples from 71 commercial alfalfa fields located in Arizona, California, Montana, Oregon, Washington, and Wyoming were assayed for susceptibility to lambda-cyhalothrin during 2020–2022 using a laboratory concentration-response assay. Seventeen field sites representing all six states were highly resistant to lambda-cyhalothrin (resistance ratios > 79.6) and bioassay mortality often did not exceed 50% even at the highest concentration tested (3.30 µg/cm2 in 2020 and 10.00 µg/cm2 in 2021–2022). Field sites assayed with more than one pyrethroid active ingredient indicated likely cross-resistance between lambda-cyhalothrin and zeta-cypermethrin (type II pyrethroids) and variable and/or limited potential cross-resistance to permethrin (type I pyrethroid). Thirty-two field sites representing five states were susceptible to lambda-cyhalothrin (resistance ratios ranging from 1 to 20). While resistance is widespread, integrated resistance management strategies including rotating mode of action groups, applying chemical control tactics only when economic thresholds have been met, and utilizing cultural control tactics can be employed to slow the further development of resistance.
Much research has explored the effects of plant species diversity on herbivore populations, but far less has considered effects of plant genotypic diversity, or how abiotic stressors, like drought, can modify effects. Mechanisms by which plant genotypic diversity affects herbivore populations remain largely unresolved. We used greenhouse studies with a model system of wheat (Triticum aestivum L.) and bird cherry-oat aphid (Rhopalosiphum padi L.) to determine whether the genotypic diversity of a plant's neighborhood influences performance and fitness of herbivores on a focal plant and if drought changes the influence of neighborhood diversity. Taken across all varieties we tested, plant-plant interactions in diverse neighborhoods reduced aphid performance and generated associational resistance, although effects on aphids depended on variety identity. In diverse mixtures, drought stress greatly diminished the genotypic diversity-driven reduction in aphid performance. Neighborhood diversity influenced mother aphid size, and appeared to partially explain how plant-plant interactions reduced the number of offspring produced in mixtures. Plant size did not mediate effects on aphid performance, although neighborhood diversity reduced plant mass across varieties and watering treatments. Our results suggest inter-varietal interactions in genotypic mixtures can affect herbivore performance in the absence of herbivore movement and that abiotic stress may diminish any effects. Accounting for how neighborhood diversity influences resistance of an individual plant to herbivores will help aid development of mixtures of varieties for managing insect pests and clarify the role of plant genotypic diversity in ecosystems.
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