Stromatolites are abundant in Upper Palaeocene to Eocene fluvial deposits from the Eastern Ebro Basin, whereas they are scarce in the lacustrine facies. The fluvial stromatolites display a variety of growth forms: oncoids, domes, laminated crusts and locally irregular bioherms. These morphologies are related to the hydrological behaviour of the fluvial systems. Elongate domes, dome heads and ovoid to subspherical oncoids accreted in flowing water. Laminated crusts and small‐sized cylindrical to lenticular oncoids formed during low‐discharge and ponding phases. In the lacustrine environments only small‐sized ovoid oncoids occur.The main characteristics of the Ebro Basin non‐marine stromatolites are: (1) laminations and concentric banding exhibited by all morphologies; (2) the occurrence of well‐preserved cyanobacterial filaments in several laminae, suggesting that these microbiotas are the major contributors in growth; (3) the asymmetrical growth of cortices in domes and oncoids with polar thickening on the upper side suggesting in situ growth under low energy conditions.δ13C values of carbonate stromatolites range from ‐ 11.3%o (Late Palaeocene) to — 4.4%o (Middle‐Late Eocene). During the Late Palaeocene, under tectonically quiescent conditions, the δ13C values mainly reflect the influence of soil‐derived CO2. During the Eocene, the progressive uplift of the surrounding catchment areas led to an increase of exposures of Mesozoic rocks. Consequently, the δ13C values of the Eocene stromatolites reflect the influence of the CO2 derived from the dissolution of Mesozoic marine carbonates.There is a 2.5%o shift in δ18O values of stromatolites from the Late Palaeocene to Middle‐Late Eocene which is consistent with the variation in δ18O of precipitation due to changes in altitude of the catchment area during this time.The isotopic values of all Middle‐Upper Eocene laminated crust samples show distinct covariant trends, suggesting that these stromatolites were formed either in ponding zones of fluvial channels or in disconnected pools developed during low‐discharge episodes.
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