The aim of this study was to determine the changes of metabolomic profiles in embryonic culture media (ECM) for the evaluation of quality and implantation potential of human embryos. ECM (n=163) were collected on day 5 before transfer or cryopreservation. Some embryos were used in preimplantation genetic screening for detection of aneuploidy karyotypes. Samples were subdivided into groups according to embryo morphological classification (by Gardner), genetic analysis and implantation data. ECM were extracted with methanol, precipitates were separated by centrifugation and metabolite production of individual embryo was analysed by LC-MS (the positive ion mode). After peak detection and retention time alignment, data were analysed using the PCA algorithm. MS fingerprinting analysis of embryo culture medium showed significant differences between morphologically divided groups. Intragroup comparisons did not reveal differences between subclasses. Genetic screening of embryos revealed 33 aneuploid karyotypes. It was shown that chromosome number did not affect the metabolite profiles comparing with the normal group. The culture media of embryos that were positive or negative for successful implantation showed specific signatures that allowed to distinguish embryos with different outcomes.The characterization of ECMs by LC-MS may facilitate more accurate selection of the best embryo for the implantation, improving single-embryo transfer and thus eliminating the risk and undesirable effects of multiple pregnancies.
Introduction:This research presents data about embryo capability for implantation based on the study of changes in metabolomic profile of embryo culture media, consumption of constituents of embryo culture media and anamnestic factors predisposing to aneuploid embryos. Determination of glucose and glutamate concentrations in spent media samples from embryos collected on day 5 is a new noninvasive method of assessment embryo quality to efficiency improvement of IVF outcomes.Material and methods: 96 couples underwent IVF program with preimplantation genetic testing (PGT). Spent media samples from embryos that resulted in pregnancy and delivery and samples from embryos that failed to implant were individually collected on day 5, and evaluated using mass-spectrometry.
Results:Сorrelation was identified between metabolomic profile, implantation potential of embryo and ploidy of embryos.
Conclusions:Analysis of composition of embryo culture media maid by method of metabolomic profiling can result in more specific selection of embryos for further transfer.
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