Integration of organic light emitting diodes (OLEDs) and organic photodetectors (OPDs) on flexible plastic substrates promises compact and low-cost optical detection units for multiplex sensors. These units may be laminated to a microfluidic system for sensing applications in a liquid. Here, a 6 × 6 element matrix of alternating blue OLEDs and OPDs is demonstrated on a single flexible plastic substrate. The devices are fabricated by masked thermal evaporation on a 200 µm thick polyethylene terephthalate (PET) foil. The individual device size is 1 mm × 1 mm. Both OLEDs and OPDs are demonstrated to work. The spectral characteristics are shown to be suitable for fluorescence measurements. Signals from fluorescence-labeled spots above the OPDs under OLED excitation are investigated. Successful operation of the OLED-OPD matrix for reflection measurement is demonstrated.
We present a system efficiency analysis of a monolithic integrated organic optoelectronic unit for the detection of fluorescence labeled single-stranded DNA (ssDNA) for veterinary disease testing. The side-by-side integration of an organic light emitting diode (OLED) and an organic photodetector (OPD) with 0.5 mm by 0.5 mm device sizes has the potential to enable compact and low-cost fluorescence point-of-care (POC) devices for decentral multiplex biomedical testing. Here, we used two 6-FAM and BHQ1 labeled complementary ssDNA strands to form the Förster resonance transfer (FRET) upon the hybridization of the DNA. In this work we successfully show ssDNA hybridization sensing with samples diluted in TE buffer and investigate the detection of covalently bound 6-FAM-ssDNA on a glass surface for multiplex biomarker measurements.
Collecting real-time data on physical and chemical parameters of the soil is a prerequisite for resource-efficient and environmentally sustainable agriculture. For continuous in situ measurement of soil nutrients such as nitrate or phosphate, a lab-on-chip approach combined with wireless remote readout is promising. For this purpose, the soil solution, i.e., the water in the soil with nutrients, needs to be extracted into a microfluidic chip. Here, we present a soil-solution extraction unit based on combining a porous ceramic filter with a microfluidic channel with a 12 µL volume. The microfluidic chip was fabricated from polydimethylsiloxane, had a size of 1.7 cm × 1.7 cm × 0.6 cm, and was bonded to a glass substrate. A hydrophilic aluminum oxide ceramic with approximately 37 Vol.-% porosity and an average pore size of 1 µm was integrated at the inlet. Soil water was extracted successfully from three types of soil—silt, garden soil, and sand—by creating suction with a pump at the other end of the microfluidic channel. For garden soil, the extraction rate at approximately 15 Vol.-% soil moisture was 1.4 µL/min. The amount of extracted water was investigated for 30 min pump intervals for the three soil types at different moisture levels. For garden soil and sand, water extraction started at around 10 Vol.-% soil moisture. Silt showed the highest water-holding capacity, with water extraction starting at approximately 13 Vol.-%.
The paper presents a data analysis of the international scientific community on the issues of vermicultivation for the last 75 years. The authors considered terminology, the biochemical contents of earthworms’ tissues, production technology; distinguished 4 stages, 8 methods and biological aspects of their safety. The paper covers economic production characteristics. The results on the practical use of dry and paste vermiproducts have been summarized: feed additives in fishing (6 examples), poultry farming (8 examples), mammal breeding (3 examples), growth media for microorganisms (1 example). The benefits of vermiproducts as food supplements for humans have been explored. Vermi food contains a high amount of protein (64.5-72.9 % per dry weight), all essential amino- and fatty acids, calcium (cheese and milk), iron (10 times more than in soybeans).
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