The results of cytogenetic and i-FISH investigations in bone marrow cells of 99 patients at diagnosis of multiple myeloma are presented. Metaphase chromosomes slides were prepared according to the generally accepted methods, after 24 and 96 h of cultivation in nutrient medium without stimulation. According to the clone structure, karyotypes with normal and normal/near tetraploid clones were dominated (78.3 %). With regard to the ploidy level, pseudodiploid clones were recorded more often (12.1 %), hyperdiploidy was revealed in 7.2 % cases. Among the structural rearrangements, the deletions were dominated, namely, 12.1 % were found by cytogenetic study and 34.5 % – by- i-FISH. The structural rearrangements were shown to involve chromosomes 13 and 17 (6 % and 1.2 %, respectively) more frequently using standard cytogenetic study, while del(17p) and del(13q) (24.1 % and 15.5 %, respectively) – by- i-FISH. Atypically low was a frequency of translocations involving the gene IgH (14q32) (1,8 %). Unusual distribution of the signals with the involvement of IgH gene indicates an additional mechanism for the abnormal clone formation. By the cytogenetic analysis data, 8.9% cases, by the i-FISH data, 34.5 % cases and summarizing both methods – 52.2 % cases, were referred to the group of adverse genetic prognosis during the course of disease.
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