Use of hydrogen peroxide (H202) under various conditions of temperature and time to inactivate aflatoxin MI in artificially contaminated raw milk was examined. The degree of inactivation was measured by Enzym Linked Immunosorbent Assay (ELISA). It appeared that there is no change in the content of aflatoxin MI in milk contained H202 and examined after 24 hours without heat treatment and in contaminated milk without add of H20, and heat treated treatment at (63°C for 30min, 75°C for 15sec) but slight inactivation (4.3 Yo) was obtained in milk boiled for 5min without H 2 0 2 . Maximum inactivation (27.8 %, 28.8 70 and 45.1 %) were obtained using 1 % H202 followed by heat treatment at 36 "C for 30 min, 75 "C for 15 sec and boiling for 5 min respectively.
A total of 1136-quarter cow's milk samples were collected from apparently healthy cow's from 2 dairy farms in El Gharbia and 3 dairy farms in Kafr El-Sheikh Governorates (Egypt). All samples were screened for subclinicl mastitis using California Mastitis Test (CMT), Somatic Cell Count (SCC), lactose and chloride content. All Positive CMT samples were examined microbiologically for isolating the most causative microbial agents of subclinical mastitis. 136 (22.5%) samples collected from El-Gharbia Governorate were positive for CMT .Of them 100 samples (73.5%) scored 2+ and 36 (26.5%) scored 3+; 120 (20%) contained lactose below 4.7% and 140 (23.2%) had chloride content greater than 0.14%. While 65 (12.2%) quarter milk samples collected from Kafr El-Sheikh farms were CMT positive, of them 24 (36.9%) samples scored 2+ and 41(63.1%) samples scored 3+ ; 72 (13.5%) samples contained lactose less than 4.7% and 119 (22.4%) had chloride content greater than 0.14%. Infectious organisms isolated from CMT positive samples were Coliforms (88.2%), Staphylococci (26.5%) and E-coli (14.7%) while both coagulase +ve Staph. aureus and Strept. agalactiae failed to be detected from El-Gharbia quarter samples. On the other hand Coliforms , Staphylococci, coagulase +v Staph. aureus , E-coli and Strept agalactiae were isolated with a percentages ranging from 1.5 to 52.3 % from Kafr El-Sheikh samples. Somatic cell count (SCC) ranged from >105 to <107 in CMT positive samples. The in vitro sensitivity pattern of the
160 random samples of natural Tilapia nilotica (NT), farmed Tilapia nilotica (FT), natural Mugil cephalus (NM) and farmed Mugil cephalus (FM) (40 of each) were collected from eight fish farms and Alborolos lake in Kafr El-sheikh governorate through 12 months. The collected samples were subjected to chemical examinations to evaluate the effect of rearing conditions and fish types on nutritional value and quality of the examined fish. The study revealed that the farming rearing conditions leads to a significant higher fat and protein percent in farmed fish than natural fish while the Mugil cephalus species were higher than tilapia nilotica in fat and protein percent.
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