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Summary:megalovirus, as well as a low incidence of graft-versushost disease. 3,8,9 The major problem of long-term UCB banking is the One of the main problems for the establishment of umbilical cord blood (UCB) banks is the storage space storage space required for unprocessed units. Several attempts have been made to reduce the UCB volume withneeded for the frozen samples. The aim of this study was to find a method of reducing the volume of UCB out loss of progenitor cells. + + + cell quantification were done in whole blood and in the isorecovery of progenitor cells using rouleaux formation induced by 6% hydroxyethyl starch and centrifugation to lated fractions. The average volume of the 19 UCB samples processed was 103 ml. Separation by centrifugreduce both erythrocytes and plasma. Most of these techniques imply the addition of exogenous materials and are ation led to a mean volume reduction of 56% with red cell depletion of 59%. The white blood cell recovery was carried out in an open system with the risk of contamination. of 72% with a significant CD34 + + + cell recovery of 87%. This seems a promising method for cord blood volumeThe aim of this study was to find a method for volume reduction of UCB by centrifugation with partial removal of reduction and enrichment of CD34 + + + cells. Keywords: cord blood processing; CD34 + cells; cord red cells and plasma without significant losses of the HPC CD34 + cells, using a closed system for collection and problood bank cessing.Bone marrow transplantation plays an important role in the Materials and methods treatment of many congenital and acquired haematologic diseases, either malignant or benign.1 The early haematopoietic progenitor cells are essential for haematologic and UCB collection bags immunologic bone marrow reconstitution and express the A triple blood collection system (R1656B; Baxter, Lisbon, surface glycoprotein CD34.2 It is generally known that Portugal) with a 450 ml bag containing citrate-phosphatehuman umbilical cord blood (UCB) is a rich source of dextrose-adenin (CPD-A) anticoagulant was used to collect haematopoietic progenitor cells (HPC-CD34 + ) and has been the UCB. The volume of CPD-A was reduced to 20 ml successfully used as a source of progenitor cells for bone transferring the excess to the SAG manitol satellite bag, marrow reconstitution. [3][4][5][6][7] which was then sealed and removed, keeping the system Until 1996 more than 100 cord blood transplants had closed. been performed in children or adults with total or partially HLA-matched donors. 8 The UCB offers several advantages over bone marrow and peripheral blood as it is available UCB collection without risk to mother or infant, easy and cheap to collect, and has decreased transmission of infection, namely cyto-UCB were collected from full-term pregnancies with informed consent from the donors following delivery and clamping of umbilical cord. UCB was collected from the umbili- were stored at 4°C and processed within the following 24 h.

Background: Graft-versus-host disease (GvHD) remains the major toxicity of allogeneic bone marrow transplantation (BMT). In the acute form of the disease, the differential diagnosis includes viral rash and drug eruptions. Methods: We report two patients with chronic myeloid leukemia submitted to allogeneic BMT who developed a severe form of acute cutaneous GvHD, with clinical and histological pictures mimicking toxic epidermal necrolysis (TEN). Results: We found a predominance of peripheral CD8+ T lymphocytes and, at the same time, studying the cellular profile of the blister fluid, just in the beginning of blister eruption, we also found a high proportion of CD8+ T lymphocytes, mainly CD8+CD57–. Conclusion: These data are in agreement with previous reports of the presence of CD8+ T cells in the blister fluid of patients with TEN, further emphasizing similar immunoinflammatory pathways in both diseases.

In order to determine the value of flow cytometric (FCM) immunophenotyping of fine-needle aspirates (FNA) in the diagnosis and classification of lymphoproliferative diseases, 61 tissue samples were studied and compared with the cytologic/histological results. In vivo and ex vivo FNA biopsy yielded the material for FCM, which comprised an extensive number of lymphoid cell markers. In all but three cases sufficient cells were collected. Overall, malignancy was diagnosed in 33 cases from a total of 47 (70.2%), and in the remaining cases malignancy was not detected. Eleven cases were correctly diagnosed as reactive processes (11/11). There were no false positive cases of malignancy, as diagnosed by FCM-FNA. The best accuracy was achieved in the low-grade B-cell lymphomas and lymphoblastic lymphoma/leukemia. We conclude that in a significant number of cases, FCM-FNA permits the separation between lymphoid malignancies and reactive processes without false positive results. It was found to be particularly useful in the differential diagnosis of mantle-cell and small-lymphocytic lymphoma and in the identification of lymphoblastic lymphoma/leukemia.