In a screening experiment with 215 different winter cukivars of Triticum aestivum L. for response in anther culture, pollen embryos or callus were obtained from 200 different cukivars and green plants from 93 different cultivars. On average, from the whole material, 1.3 green plants were obtained per 100 anthers cultured. Variance components estimated from replicates with selected parts of the plant material indicated that for the formation of pollen embryos from anthers, interactions between genotypes and replications were dominating, accounting for 45 to 50 per cent of the variation. Main effects from genotypes were less prominent, accounting for 25 to 30 per cent of the variation, and the replications showed relativel)' little effect, accounting for about 4 to 12 per cent of total variation. Regeneration of plants from pollen embrj'os or callus was not significantly mfluenced by either genotype or replicates. The frequencies of plants regenerated being green, however, were influenced from hoth genotype and environments contributing 42.5 and 34.9 per cent of the total variation respectively, while only minor interactions between genotype and environments were indicated for this character.

Anther culture response with 17 widely-grown varieties and one model variety of barley was studied witb one replication from field-grown donor plants and one replication from a growth-chamber. Plants were regenerated from all 18 varieties and green plants were obtained from 16 of them. On average, 1.6 green plants were obtained per 100 cultured anthers from all the material. Estimated variance components for the formation of embryos/callus from tbe anthers were dominated by the effects of the genotypes and interactions between plant material and environments which together accounted for 60.1 and 17.0 % of the total variation respectively, while environments were nonsignificant for this character. Plant regeneration from enibryos/calkis were not significantly influenced by either genotype or environments. Components of variance for green plant formation were dominated by the effects of the genotypes, accounting for 73.2 % of the total variation, and a smaller effect from environments accounting for 11.2% of the total variation. Mam effects from genotypes on the percentage of green regenerants divided 7 varieties into two distinct groups, indicating that nia)or genetic factors were involved. The genetic basis for green plant regeneration seems different from that governing embryo formation. The results are discussed with respect to the possible prediction of anther culture response for new barley hybrids, as a means for directing the use of barley anther culture towards material that responds well.

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lhe segregaiion ol isozyme markers has been examined in lhe ,t;reen and alliino andt-ogenelie progenies ot ivvo plants ol Lolium perenne. Overall the pooled data tor tbe loei P(JI/2 atid G'O7'/2 iloes not deviate Irom expeetation, but there is eonsiderahle heterogeneity between t.unihes and between green ami albino plains, S[iecilie alieles predominate in the diltereni progenv sets, Ihe results mav' be accounted lor by possible linked loci allecting anther culture response or ol linked lethal i^enes.

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