We present first results of Hipparcos observations of nearby low‐mass pre‐main‐sequence (PMS) stars (T Tauri and Herbig Ae/Be stars). The data obtained by Hipparcos allow us to derive weighted mean parallaxes for three major nearby star‐forming regions (SFRs), Lupus, Chamaeleon I and Taurus–Auriga. Furthermore, results on the isolated objects AB Dor and TW Hya are presented. Finally, we discuss the evolutionary status of Herbig Ae/Be (HAEBE) stars on the basis of Hipparcos results.

Abstract.We have extensively monitored the Luminous Blue Variable AG Car (HD 94910) spectroscopically. Our data cover the years 1989 to 1999. In this period, the star underwent almost a full S Dor cycle from visual minimum to maximum and back. Over several seasons, up to four months of almost daily spectra are available. Our data cover most of the visual spectral range with a high spectral resolution (λ/∆λ ≈ 20 000). This allows us to investigate the variability in many lines on time scales from days to years. The strongest variability occurs on a time scale of years. Qualitatively, the variations can be understood as changes of the effective temperature and radius, which are in phase with the optical light curve. Quantitatively, there are several interesting deviations from this behaviour, however. The Balmer lines show P Cygni profiles and have their maximum strength (both in equivalent width and line flux) after the peak of the optical light curve, at the descending branch of the light curve. The line-width during maximum phase is smaller than during minimum, but it has a local maximum close to the peak of the visual light curve. We derive mass-loss rates over the cycle from the Hα line and find the highest mass loss rates (logṀ /(M yr −1 ) ≈ −3.8, about a factor of five higher than in the minimum, where we find logṀ/(M yr −1 ) ≈ −4.5) after the visual maximum. Line-splitting is very commonly observed, especially on the rise to maximum and on the descending branch from maximum. The components are very long-lived (years) and are probably unrelated to similar-looking line-splitting events in normal supergiants. Small apparent accelerations of the components are observed. The change in radial velocity could be due to successive narrowing of the components, with the absorption disappearing at small expansion velocities first. In general, the linesplitting is more likely the result of missing absorption at intermediate velocities than of excess absorption at the velocities of the components. The Hei lines and other lines which form deep in the atmosphere show the most peculiar variations. The Hei lines show a central absorption with variable blue-and red-shifted emission components. Due to the variations of the emission components, the Hei lines can change their line profile from a normal P Cyg profile to an inverse P Cyg-profile or double-peak emission. In addition, very broad (±1500 km s −1 ) emission wings are seen at the strongest Hei lines of AG Car. At some phases, a blue-shifted absorption is also present. The central absorption of the Hei lines is blue-shifted before and red-shifted after maximum. Possibly, we directly see the expansion and contraction of the photosphere. If this explanation is correct, the velocity of the continuum-forming layer is not dominated by expansion but is only slightly oscillating around the systemic velocity.

Brucella spp. were isolated from an abortion case submitted for laboratory examination 8 months after the first clinical symptoms appeared in a kennel consisting of 31 dogs. Pathological investigations revealed the parallel presence of necrotic placentitis and the strong immunostaining of trophoblast cells by immunohistochemistry (IHC) using hyperimmune rabbit anti-Brucella canis primary antibodies. The rapid slide agglutination test was positive in 7 of 31 (23%) cases. The organism B. canis was successfully cultured from the blood, tissues, or vaginal swabs of only 3 of 31 (10%) cases. The isolated strains were identified as B. canis based on their colony morphology and agglutination with R sera. The strains were initially misidentified as B. suis with the "Bruce-ladder" method, and were subsequently correctly identified as B. canis with a single nucleotide polymorphism (SNP) typing test. Three culture-positive cases and 3 culture-negative cases with histories of reproductive disorders were selected and examined for the presence of B. canis infection using histopathology, IHC, and polymerase chain reaction (PCR) assays. Characteristic histologic lesions were found in all of the 6 animals, whereas IHC and PCR yielded positive results only in single cases from both groups. The results imply that all cases of canine abortion should be examined for brucellosis by bacterial culture of aborted fetuses and placentas. Immunohistochemical examination of placentas is also recommended because it is a quick and sensitive technique compared with bacterial culture. Multiple methods (i.e., serology, blood, and genital bacterial cultures) should be applied simultaneously and repeatedly for the reliable screening of B. canis infection in live individuals.

The plasmid types and serotypes of 164 Rhodococcus equi strains obtained from submaxillary lymph nodes of swine from different piggeries in 28 villages and towns located throughout the country were examined. The strains were tested by PCR for the presence of 15-to 17-kDa virulence-associated protein antigen (VapA) and 20-kDa virulence-associated protein antigen (VapB) genes. R. equi is an aerobic, gram-positive coccobacillus which frequently causes chronic suppurative bronchopneumonia with abscesses, lymphadenitis, and ulcerative enteritis in foals less than 6 months old (3,14). In addition to its virulence for foals, R. equi seems also to be an important pathogen to immunocompromised humans, such as organ transplant and AIDS patients (21). R. equi is also common in the submaxillary lymph nodes of pigs (2,8,15,25,27). Katsumi and others (8) isolated R. equi from 45.6% of the submaxillary lymph nodes of swine with lesions and from 9.4% of lymph nodes of swine without lesions. Takai and colleagues (19) described a 3.1% isolation rate on the basis of examination of 1,832 submaxillary lymph nodes collected from swine.Recently, the discovery of virulence-associated antigens and virulence plasmids has allowed classification of the virulence of More recently, we demonstrated that five of the seven clinical isolates of R. equi from immunocompromised patients expressed VapB and that they were of intermediate virulence and revealed that these human isolates contained a 95-kb type 5 plasmid which was also seen in the pig isolates in Hungary (10). The route of infection in human cases is not clear. The purpose of this study was to isolate virulent R. equi strains from submaxillary lymph nodes of swine in Hungary, to determine the genotypic diversity of virulence-associated plasmids found in them, and to examine the serotypes of the isolates with the aim of finding additional data to characterize the epidemiological relationship between human R. equi infections and pigs carrying R. equi in the submaxillary lymph nodes.Serotyping is a reliable method for examining R. equi strains. There are two systems for serotyping R. equi. Prescott de-* Corresponding author. Mailing address:

Background Poxviruses are large DNA viruses that infect humans and animals. Vaccinia virus (VACV) has been applied as a live vaccine for immunization against smallpox, which was eradicated by 1980 as a result of worldwide vaccination. VACV is the prototype of poxviruses in the investigation of the molecular pathogenesis of the virus. Short-read sequencing methods have revolutionized transcriptomics; however, they are not efficient in distinguishing between the RNA isoforms and transcript overlaps. Long-read sequencing (LRS) is much better suited to solve these problems and also allow direct RNA sequencing. Despite the scientific relevance of VACV, no LRS data have been generated for the viral transcriptome to date. Findings For the deep characterization of the VACV RNA profile, various LRS platforms and library preparation approaches were applied. The raw reads were mapped to the VACV reference genome and also to the host ( Chlorocebus sabaeus ) genome. In this study, we applied the Pacific Biosciences RSII and Sequel platforms, which altogether resulted in 937,531 mapped reads of inserts (1.42 Gb), while we obtained 2,160,348 aligned reads (1.75 Gb) from the different library preparation methods using the MinION device from Oxford Nanopore Technologies. Conclusions By applying cutting-edge technologies, we were able to generate a large dataset that can serve as a valuable resource for the investigation of the dynamic VACV transcriptome, the virus-host interactions, and RNA base modifications. These data can provide useful information for novel gene annotations in the VACV genome. Our dataset can also be used to analyze the currently available LRS platforms, library preparation methods, and bioinformatics pipelines.

A 1-year study of the ecological cycle of Francisella tularensis was performed in an enzootic area during an inter-epizootic period. The study was based on multiple sampling of all major constituents of the disease cycle. Seroprevalence of tularemia in the European brown hare (Lepus europaeus) population was 5.1% (10/197) with low antibody titers (1/10 and 1/20), and F. tularensis ssp. holarctica was isolated from four hares. F. tularensis was not detected in the 38 common voles (Microtus arvalis), 110 yellow-necked mice (Apodemus flavicollis), or 15 stripped field mice (Apodemus agrarius) trapped during the study, or the by-catch of 8 Eurasian pygmy shrews (Sorex minutus) or 6 common shrews (Sorex araneus). A total of 1106 Ixodes ricinus and 476 Haemaphysalis concinna ticks were collected from vegetation, and 404 I. ricinus, 28 H. concinna ticks, and 15 Ctenophtalmus assimilis and 10 Nosopsyllus fasciatus fleas were combed off small mammals. One H. concinna female and one nymph collected from the vegetation was found infected with F. tularensis ssp. holarctica by TaqMan polymerase chain reaction, thus resulting a 0.42% (2/476) prevalence. F. tularensis-specific DNA was not detected in environmental water samples, and the examined 100 sheep, 50 cows, and 50 buffalos grazed at the study area were all seronegative. During inter-epizootic periods, F. tularensis ssp. holarctica seems to persist only in the European brown hare--H. concinna cycle at the studied habitat. H. concinna may not serve exclusively as an arthropod vector, but it may also harbor bacteria for 3-4 years through multiple life stages and act as an important reservoir of F. tularensis. Rodent species probably do not serve as true reservoir hosts of tularemia.

No abstract

Using full-sky imaging polarimetry, we measured the celestial distribution of polarization during sunset and sunrise at partial (78% and 72%) and full (100%) moon in the red (650 nm), green (550 nm), and blue (450 nm) parts of the spectrum. We investigated the temporal change of the patterns of degree p and angle α of linear polarization of sunlit and moonlit skies at dusk and dawn. We describe here the position change of the neutral points of sky polarization, and present video clips about the celestial polarization transition at moonlit twilight. We found that at partial moon and at a medium latitude (47° 15.481' N) during this transition there is a relatively short (10-20 min) period when (i) the maximum of p of skylight decreases, and (ii) from the celestial α pattern neither the solar-antisolar nor the lunar-antilunar meridian can be unambiguously determined. These meridians can serve as reference directions of animal orientation and Viking navigation based on sky polarization. The possible influence of these atmospheric optical phenomena during the polarization transition between sunlit and moonlit skies on the orientation of polarization-sensitive crepuscular/nocturnal animals and the hypothesized navigation of sunstone-aided Viking seafarers is discussed.