Isolates of Erwinia sp., Bacillus sp., B. pumilus, B. brevis, Clavibacter sp. was recovered from stems, flowers, bolls, and roots of DP41 at average sp., and Xanthornonas sp. were recovered from surface-sterilized radicles, reisolation frequencies of 97, 82, 77, and 48%, respectively, after its introroots, stems, unopened flowers, and bolls of greenhouse-and field-grown duction into germinated seeds. Reisolation frequencies of an antibioticcotton plants of cultivars Delta Pine 41 (DP41) and Delta Pine 61 (DP61). resistant Bacillus sp. from the above tissues were 34, 12, 5, and 0%, These bacteria could not be eliminated from seeds and the above-ground respectively. In contrast, antibiotic-resistant mutants of B. pumilus, B. organs by stringent surface sterilization methods. Erwinia sp. was the brevis, Clavibacter sp., and Xanthornonas sp. could not be reisolated most prevalent bacterium with an average isolation frequency of 69% from these same organs. Similar results were obtained with DP61. The for DP41 and 51% for DP61. An antibiotic-resistant mutant of Erwinia recovered endophytes were not pathogenic to the two cultivars.