Sardinella lemuru is a small pelagic fish that contains high omega-3 which is distributed in the eastern Indian Ocean including the Bali Strait. Lemuru including high-fat with omega-3 content. This different fat content depends on the size of the fish, maturity, season, and type of food. The research objectives were to obtain abundance and diversity of microalgae composition as food and determine the level of omega-3 in the stomach of lemuru. The research was conducted at the 8 station with different waters characteristic. The data obtained were analized using t-test. The omega 3 content of lemuru in the second transitional season was 35.25% higher than the fish caught in the western season (21.4%). Lemuru feed on phytoplankton dominated by the Bacillariophyceae group with Chaetoceros gracilis in the second transitional season. These phytoplankton suspected important role as raw materials for forming omega 3 in the body of lemuru.
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