It is considered that renewal of growth in serotoninergic caudal fibers their sprouting to injury site and formation of outgrowth net is factor for locomotion recovery. The aim of our work was to study the influence of rat fetal neural cells enriched with serotonin-producing cells, on morphogenesis, gene expression of serotogenesis (Pet1, Nk2.2, Lmx1b, Tph1, Tph2, Sert) and serotonin level in organotypic n. raphe cultures in injury of neurite-glial fibers in vitro. Injury was modeled by the way of crossing neurite-glial fibers after their formation under long-term culture. The cell accumulation was observed in the injury zone when fetal n. raphe cells, previously enriched with serotonin-producing cells were added into organotypic culture of n. raphe with crossing fibers under the culture conditions. As a result, crossing site was filled with these cells after 14 days of culture. The activation of expression in cascade of regulatory genes-regulators of serotogenesis Pet1, Lmx1b, Tph1, Tph2, Sert and increased serotonin content in the culture material were also observed. The activation of gene expression of serotogenesis Pet1 and Sert, and increased serotonin content by 1.7 times were revealed when conditioned medium from culture of fetal rat cells was used. An eviction of single cells was observed in control samples of organotypic n. raphe culture after injury. Crossing site was remained without signs of cell filling in these samples at day 14 after culture. The activation of regulatory gene expression of serotogenesis Pet1, Nkx2.2, Lmx1b, Tph1, Tph2, Sert and restoration of serotonin content were also absent in the culture material.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.