Arachidonic acid causes an increase in free cytoplasmic calcium concentration ([Ca2+]i) in differentiated skeletal multinucleated myotubes C2C12 and does not induce calcium response in C2C12 myoblasts. The same reaction of myotubes to arachidonic acid is observed in Ca2+-free medium. This indicates that arachidonic acid induces release of calcium ions from intracellular stores. The blocker of ryanodine receptor channels of sarcoplasmic reticulum dantrolene (20 µM) inhibits this effect by 68.7 ± 6.3% (p < 0.001). The inhibitor of two-pore calcium channels of endolysosomal vesicles trans-NED19 (10 µM) decreases the response to arachidonic acid by 35.8 ± 5.4% (p < 0.05). The phospholipase C inhibitor U73122 (10 µM) has no effect. These data indicate the involvement of ryanodine receptor calcium channels of sarcoplasmic reticulum in [Ca2+]i elevation in skeletal myotubes caused by arachidonic acid and possible participation of two-pore calcium channels from endolysosomal vesicles in this process.
The aim of this article is the improvement of the theoretical bases and the development of a scientifically grounded approach balancing the food supply from the economic and the practical application for a complex social and economic assessment of development of the food sphere as a whole. Design/Methodology/Approach: In the process of research the authors' approach was used to consider the balance of food supply from the position of three structural and consistently interrelated elements-phenomena, process and state. The authors' interpretation of each element is given, the method of their evaluation is developed, based on the structural division of indicators into balanced ones, exceeding the level of balance and being below the specified level. These indicators are differentiated into 7 functional groups (from the minimum to the maximum degree of balance). Findings: The result is a comparative assessment of the level of balance of food supply in the UK, France, Czech Republic, Latvia and Russia and the definition on its basis of priority directions of development of agriculture in each of the allocated states. Practical Implications: The results of the work can be used by governing bodies to make specific practical decisions in the food sector, and to adjust the directions of its development. Originality/Value: Materials of the article are original and have not been previously published. The calculations were based on official data from the European Statistical Agency, Eurostat and the Federal State Statistics Service of Russia, which indicates the reliability of the presented scientific materials.
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