Previous histological studies of cyanoacrylate in wound healing have all used Oil-Red-O staining of paraffin sections prepared by routine method. In the course of our studies we began to suspect that artifact was being introduced because of dissolution of cyanoacrylate during processing. Accordingly, biopsis of wounds sealed with cyanoacrylate and pieces of cyanoacrylate of a standard known dimension with no associated tissue were observed after every stage of histological preparation. It was observed that approximately 80% of the cyanoacrylate was lost at the deparaffinization in xylene stage. Accordingly, a number of solvents were tested, and it was found that petroleum ether could be used to remove paraffin completely without the loss of any of the cyanoacrylate from the specimen. This technique has been used to view the location and ultimate fate of cyanoacrylate applied to wounds and examined at different stages in healing process. It is concluded that previous histological studies of cyanoacrylate in wound healing have been inaccurate due to leaching out of most of the tissue adhesive during deparaffinization of the specimen.
Spiramycin was tested as a chemotherapeutic plaque control agent. Sixty‐ three volunteers (29 experimental and 34 control) abstained from mechanical oral hygiene procedures for 11 weeks. The participants were divided randomly into exeperimental and control groupa and received, on a double blind basis, either 20–500 mg capsules of spiramycin or 20 capsules of placebo with directions to take 1 capsule 4 times a day for 5 days. At each examination visit intra‐oral photographs were taken, gingival and plaque indices recored, and plaque samples collected for laboratory study. In the experimental group there was a statistically significant decrease in plaque as measured by wet weight, turbidity, nitrogen, and carbogydrat parameters for at least 3 weeks. There was a significant decrease in the number of Streptococcus mutasn and S. sanguis in the plaque samples at weeks 1 and 3, but there was no detectable influence on the number of Gram‐negative organisms.
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