Analysis of the complete genome sequence of Bacillus subtilis has identified the gene yvgW encoding a protein of 703 amino acids with sequence similarity to the cadmium resistance determinant CadA from the Staphylococcus aureus plasmid pI258. Deletion of yvgW (designated cadA) resulted in increased sensitivity of the strain to cadmium. The cadA gene is expressed from its own promoter, and its expression is induced by cadmium. Northern hybridization analysis showed that cadmium induces the synthesis of a 2.2-kb cadA transcript. These results indicate that cadA is the chromosomal determinant to cadmium resistance in B. subtilis. ß
Abstract. The paper is devoted to the questions of assessment and analysis of the energyinfrastructural potential of Russian regions. The characteristics of energy infrastructure in a region play an important role in the evaluation of investment attractiveness of potential places for industrial investment. This paper describes the author's methodical approach to the assessment and analysis of the energy-infrastructural potential of the regions by plotting energy-infrastructural maps and by building a matrix of the regions. It allows distinguishing similar territorial entities based on the indicators of energy infrastructure with the aim of increasing the efficiency of the selection procedure for potential places for industrial investment. The developed method was tested on the example of assessment and analysis of characteristics of Russian region's energy infrastructure. That resulted in the construction of rating of the regions by the level of energy-infrastructural potential and generating of energy-infrastructural maps. The obtained results have theoretical and practical importance and can be used in the development of activities in the field of energy efficiency in the regional context and in the analysis of the energy-infrastructural capacity of potential locations for industrial investments. Keywords: energy-infrastructural potential, investment attractiveness, the ratio of regions, the coefficient of energy tariffs, the coefficient of technological connection, industrial investment, the region's economy, energy infrastructure, energy tariffs, technological connection.
Trichinellosis is a parasitic disease that affects animals and humans. Trichinella is a causative agent seen as a small round worm invisible to the eye. Trichinellosis affects pets and wild animals. Pigs, horses, dogs and synanthropic rats are seen to suffer from the disease more often among the domestic animals, and bears, wild boars, foxes, badgers and others – among the wild ones. . Human infection takes place when eating meat and meat products as raw dried homemade sausages and ham, kebabs, fried meat and other meat products contaminated with trichinell larvae. Infestation of wild animals is caused by predation or eating of dead animals. Pets are infected by eating slaughter products, food scraps and dead animals (rats). Trichinella are preserved in the animal muscles for some years. Badgers populations are seen as one of trichinosis reserves in Amur region. To determine specific features of trichinella larvae distribution in the lean tissue, the researchers explored the materials of 21 badgers from different areas of Amur region. The researchers used the heads or separate muscle groups for conducting the research. This is explained by remote location of many districts from the regional center. Trichinella larvae were detected by compressor trichinelloscopy and digestion in artificial gastric juice. The analysis showed that the number of trichinell larvae in the same muscle group does not depend on the side of the animal’s body, i.e. their number is almost identical on both the left and right sides. Invasion rate (IR) was defined as ratio of the number of infected animals to the total number of animals explored (in percentage). Invasion intensity (II) was determined by the number of trichinell larvae in 1 g of lean tissue (lye/g). The same method was used to investigate the distribution of trichinella larvae in 15 muscle groups of a badger. Invasion intensity in the infected animals was 14.3%. The largest number of trichinella larvae in a badger is concentrated in the head muscles, and there are no significant differences from the body part. The authors recommend to explore the badger carcasses and muscle sampling mainly from the head.
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