Consideration is given to the possibility of using LED light sources with different ratios of red (650 - 660 nm), orange (600 - 610 nm) and blue (440 - 450 nm) light in the light flux in the rooting stage of garden strawberry (Fragaria×ananassa(Duch. ex Weston) Duch. ex Rozier) grownin vitro. The study covered a sizable diversity of strawberry cultivars (selected from thein vitrocollection): ‘Borovitskaya’, ‘Vityaz’, ‘Zenga Zengana’, ‘Khoney’, ‘Tsaritsa’, ‘Kubata’, ‘Dukat’, ‘Marmalada’, ‘Valenta’, ‘Rusich’, ‘Krasavitsa Zagorya’, ‘Neznakomka’ and ‘Roksana’, thus ensuring quite diversified results. It was established that the use of LED sources with an increased content of red light in the light flux increased the rooting percentage (1.15-1.25 times) and the number of roots per one microcutting (1.29-1.77 times) inin vitroculture, as compared with fluorescent lamp lighting. It was observed that with different spectral compositions of light the rooting ability of microcuttings was cultivar-specific.
In vitro culture at the rooting stage the aft ereff ect of the use of earlier (during proliferation) growth regulators with cytokinin activity (6-benzylaminopurine and thidiazuron) and the eff ect of lamps of diff erent spectral composition on the percentage of rooting and biometric parameters of the development of the roots of clonal apple rootstocks were studied. The following forms of apple clonal rootstocks were taken as objects of research: 54-118, 57-490, 57-545, ММ 106 and М 26. The research methods used at the stages of in vitro culture studies corresponded to those generally accepted for this section of research. Studies were performed on two variants of explants, one part was cultured at the proliferation stage on media with 6-benzylaminopurine and the other on media with tidiazurone. It was found that explants that were cultivated at the stage of proliferation on media with thidiazuron had a tendency to a subsequent increase in the number of roots (at the stage of rooting) by one microscopic cut, in comparison with cultivation on media with 6-benzylaminopurine, by 1.1-1.3 times, and this regularity was observed in all studied clonal rootstocks of the apple tree. It was noted that when explants were illuminated, explants had a greater percentage of rooting when illuminated with phytolamps in comparison with lighting with lamps with a color temperature of 6500K by 1.1-1.4 times in all studied apple rootstocks, and an increase in the number of roots per one microstalk was observed (for excluding rootstock 57-490), by 1.1-1.9 times. During the research, the specificity of rooting of clonal apple rootstocks was observed under the infl uence of the composition of light and the use of 6-benzylaminopurine and thidiazuron at the stage of proliferation, which was expressed in a higher ability to rhizogenesis of some rootstocks compared to others. The rootstocks MM 106 (83.3-93.9 %) and M26 (87.8-92.7 %) had a greater percentage of rooting, according to the average number of roots per one microcut, rootstocks 57-545 (15.4 pcs.), MM 106 (11.2 pcs.), and M 26 along the average root length (27.0 mm).
Резюме. Установлено, что использование люминесцентных ламп с повышенным содержанием красного света в световом потоке положительно влияет на укоренение земляники садовой (Fragaria ananassa D.) в культуре in vitro в сравнении с освещением люминесцентными лампами других спектральных составов. Ключевые слова: клональное микроразмножение, земляника (Fragaria ananassa D.), укоренение, спектральный состав света. Summary. It has been established that the use of fl uorescent lamps with an increased content of red light in the light fl ux positively infl uences the rooting of strawberry (Fragaria ananassa D.) in in vitro culture in comparison with the illumination by fl uorescent lamps of other spectral compositions.
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