In contrast to the conventional ‘grafting’-based thermoresponsive cell culture platform, we first developed a bulk form of thermoresponsive cell culture platform for attaching/detaching diverse types and origins of the cell sheets in different shape.
Until today, the cell sheet engineering-based technologies have actualized diverse scaffold-free bio-products to revitalize unwillingly damaged tissues/organs including cardiomyopathy, corneal defect, and periodontal damage. Although a substantial interest is now...
Cell migration is
crucial in physiological and pathological processes such as embryonic
development and wound healing; such migration is strongly guided by
the surrounding nanostructured extracellular matrix. Previous studies
have extensively studied the cell migration on anisotropic nanotopographic
surfaces; however, only a few studies have reported cell migration
on isotropic nanotopographic surfaces. We herein, for the first time,
propose a novel concept of adherable area on cell migration using
isotropic nanopore surfaces with sufficient nanopore depth by adopting
a high aspect ratio. As the pore size of the nanopore surface was
controlled to 200, 300, and 400 nm in a fixed center-to-center distance
of 480 nm, it produced 86, 68, and 36% of adherable area, respectively,
on the fabricated surface. A meticulous investigation of the cell
migration in response to changes in the constrained adherable area
of the nanotopographic surface showed 1.4-, 1.5-, and 1.6-fold increase
in migration speeds and a 1.4-, 2-, and 2.5-fold decrease in the number
of focal adhesions as the adherable area was decreased to 86, 68,
and 36%, respectively. Furthermore, a strong activation of FAK/Rac1
signaling was observed to be involved in the promoted cell migration.
These results suggest that the reduced adherable area promotes cell
migration through decreasing the FA formation, which in turn upregulates
FAK/Rac1 activation. The findings in this study can be utilized to
control the cell migration behaviors, which is a powerful tool in
the research fields involving cell migration such as promoting wound
healing and tissue repair.
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