Bacterial chondronecrosis with osteomyelitis (BCO) is a major cause of lameness in broiler chicken, and results in serious economic losses worldwide. Although the pathogenesis mechanism leading to lameness is not entirely understood, some strains of
Enterococcus
sp., avian pathogenic
Escherichia coli
or
Staphylococcus aureus
have been long recognized as important causative pathogens. To prevent the progression of
Enterococcus
sp., avian pathogenic
E. coli
or
S. aureus
infections, we developed rapid, sensitive and convenient diagnostic assays using loop-mediated isothermal amplification (LAMP). Entero-Common-LAMP assays were developed for simultaneous detection of eight
Enterococcus
species. To target specific microorganisms, seven Entero-Specific-LAMP assays for
E. faecalis
,
E. faecium
,
E. hirae
,
E. gallinarum
,
E. avium
,
E. durans
and
E. cecorum
were developed, as well as
E. coli
-LAMP and
S. aureus
-LAMP assays. Considering the prevalence and economic impact of
Enterococcus
sp.,
E. coli
and
S. aureus
, the 10 different LAMP assays which were developed have considerable potential as routine diagnostic methods in the field or in resource-limited environments.
Bacterial chondronecrosis with osteomyelitis (BCO) is a major cause of lameness in broiler chicken, and results in serious economic losses worldwide. Although the pathogenesis mechanism leading to lameness is not entirely understood, some strains of Enterococcus sp., avian pathogenic Escherichia coli, or Staphylococcus aureus have been long recognized as important causative pathogens. To prevent the progression of Enterococcus sp., avian pathogenic E. coli, or S. aureus infections, we developed rapid, sensitive, and convenient diagnostic assays using loop-mediated isothermal amplification (LAMP). Entero-Common-LAMP assays were developed for a simultaneous detection of eight Enterococcus species. To target specific microorganisms, seven Entero-Specific-LAMP assays for E. faecalis, E. faecium, E. hirae, E. gallinarum, E. avium, E. durans and E. cecorum, and E. coli-LAMP and S. aureus-LAMP assays, were developed. Considering the prevalence and economic impact of Enterococcus sp., E. coli, and S. aureus, the developed ten different LAMP assays have a considerable potential as routine diagnostic methods in the field or in resource-limited environments.
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