The hydrolysis kinetics of p-nitrophenyl acetate by basic polypeptides with a variety of amino acid residues were analyzed. The results suggest that the tertiary structure of microenvironment brought about by random coil assembly is more important as a binding site than the number of basic residues (catalytic sites).
Abs~a~Phosphodiester cleavage of BNPP can be accelerated by various degradative receptors including enzymes. In the hydrolytic cleavage of BNPP, a bell-shaped receptor concentration-rate profile composed of a positive-catalysis phase at low receptor concentrations and a negative-catalysis phase at excess receptor concentration was observed. The negative catalysis can be explained by nonproductive binding, leading to an increase of the activation energy barrier and a decrease of the reaction rate.
The nonenzymatic hydrolysis of RNA by polyvinylpyrrolidone (PVP) has beea investigated. A bell-shaped kinetics was observed when the first order rate constant of the reaction was ploted as a function of PVP concentration, which means the kinetic feature of PVP as a degradative receptor distinct fxom those of degradative enzymes (e.g., ribonuolease).
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