Purpose To investigate the effects of pine needle extract (PNE) on the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 during liver regeneration induced by 70% partial hepatectomy (PH) in rat. Methods Forty-eight male rats (SD, 7 weeks) had surgery (70% PH). They were randomly divided into two groups. PH + PNE group was only provided PNE diluted in water (10%) for drinking and PH group was provided water from 5 days before surgery to the time of sacrifice. PNE was made by pressing and filtering. Animals were sacrificed at 12h, 24h, 36h, 60h, 84h, 168h after PH, respectively. The expressions of PCNA and Ki-67 were determined as proliferation indices. Results Immunohistochemistry turned out to increase the expression of PCNA and Ki-67. PCNA expression of PH+PNE group increased up to twice of that of PH group. Western blot also seemed to increase the PCNA expression. These results indicated the promotion of cell proliferation in liver tissue and hepatic regeneration. Conclusions Pine needle extract stimulates the expression of some mitotic proteins during liver regeneration induced by 70% PH in rats. It suggests that administration of pine needle extract could accelerate the liver regeneration after partial hepatectomy.
Objective: This study was carried out to evaluate the antioxidant activity and effect of a hot water extract of Lycium chinense fruit (LFE) on liver regeneration induced by Partial Hepatectomy (PH) in rats. Methods: Antioxidant activity of LFE was determined. Rats were divided into three groups; laparotomized (sham, n=15), 70% PH (control, n=25), and LFE-treated after PH (experimental, n=35). Starting 7 days before PH, rats received DW or LFE (500 mg/kg in DW) orally once a day until sacrifice. Rats were sacrificed on d 0.5, 1, 2, 3, and 7 after surgery. Liver regeneration ratio and Proliferating Cell Nuclear Antigen (PCNA) expression were determined through immunohistochemistry and western blotting. Results: Final extraction yield of LFE was 36.0%. LFE scavenged the DPPH and the ABTS radicals in a concentration-dependent manner. The IC 50 of LFE on DPPH and ABTS antioxidant activity was 5.97 mg/ml and 2.53 mg/ml, respectively. The extent of liver regeneration seen in the experimental group (91.29%) at 7 d after PH was greater than that in the control group (89.43%). After PH, the liver reconstruction process involving collapse, re-arrangement and re-modelling of hepatic tissue was observed to happen sooner in the experimental group. The number of PCNA-positive hepatocytes obviously increased in the experimental group. PCNA-positive hepatocyte ratio peaked at 2 and 3 d in the experimental and control groups, respectively. Conclusions: Administration of LFE had beneficial effects on the regenerative capacity of remnant liver tissue after 70% PH in rats, probably owing to its antioxidant and proliferative properties.
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