Nigella sativa or black seed is used as a medicinal plant around the globe. Oil and seeds have a long tradition of folklore use in various medicinal and food systems. The conventional therapeutic use of Nigella sativa, in different ways, has been reported in several studies to treat different diseases including influenza, headache, hypertension, diabetes, inflammation, eczema, fever, cough, asthma, bronchitis, and fever. Based on previously reported potential therapeutic uses of N. sativa compounds, and keeping in mind the dire need of time for the development of potent antiviral, a combined docking, ADMET properties calculation, molecular dynamics, and MM-PBSA approaches were applied in the current study to check the therapeutic potentials of N. sativa chief constituents against COVID-19. Among the studied compounds, we found that dithymoquinone (DTQ), with binding affinity of À8.6 kcal/mol compared to a positive control (chloroquine, À7.2 kcal/mol) , has the high potential of binding at SARS-CoV-2:ACE2 interface and thus could be predicted as a plausible inhibitor to disrupt viral-host interactions. Molecular dynamics simulation of 100 ns well complemented binding affinity of the compound and revealed strong stability of DTQ at the docked site. Additionally, MM-PBSA also affirms the docking results. Compound DTQ of the present study, if validated in wet lab experiments, could be used to treat COVID-19 and could serve as a lead in the future for development of more effective natural antivirals against COVID-19.
Helicobacter cinaedi is a Gram-negative bacterium from the family Helicobacteraceae and genus Helicobacter. The pathogen is a causative agent of gastroenteritis, cellulitis, and bacteremia. The increasing antibiotic resistance pattern of the pathogen prompts the efforts to develop a vaccine to prevent dissemination of the bacteria and stop the spread of antibiotic resistance (AR) determinants. Herein, a pan-genome analysis of the pathogen strains was performed to shed light on its core genome and its exploration for potential vaccine targets. In total, four vaccine candidates (TonB dependent receptor, flagellar hook protein FlgE, Hcp family type VI secretion system effector, flagellar motor protein MotB) were identified as promising vaccine candidates and subsequently subjected to an epitopes’ mapping phase. These vaccine candidates are part of the pathogen core genome: they are essential, localized at the pathogen surface, and are antigenic. Immunoinformatics was further applied on the selected vaccine proteins to predict potential antigenic, non-allergic, non-toxic, virulent, and DRB*0101 epitopes. The selected epitopes were then fused using linkers to structure a multi-epitopes’ vaccine construct. Molecular docking simulations were conducted to determine a designed vaccine binding stability with TLR5 innate immune receptor. Further, binding free energy by MMGB/PBSA and WaterSwap was employed to examine atomic level interaction energies. The designed vaccine also stimulated strong humoral and cellular immune responses as well as interferon and cytokines’ production. In a nutshell, the designed vaccine is promising in terms of immune responses’ stimulation and could be an ideal candidate for experimental analysis due to favorable physicochemical properties.
Aim: To determine the prevelance and risk factors of gastroesophageal reflux disease among the study population Study design: A cross-sectional study Place and Duration: This study was conducted at Poonch Medical College Rawalakot AJK Pakistan from January 2020 to January 2021. Methodology: This study was conducted on a sample size of 302 people utilizing a researcher-created checklist and questionnaire. The survey was made available on the internet. The data was analyzed using SPSS version 22, which comprised the Chi-square test and descriptive statistics. Questions about increased pain intensity, food intake or not, and other signs and symptoms of reflux and factors that could increase chances of risk such as drinking coffee, smoking, spicy food, certain types of drugs, and alcohol were determined. We also inquired as to whether any investigations had been conducted. Results: The cumulative prevalence of GERD among the individuals was 61.7 percent. Significant discomfort was seen in 12 percent of the cases. Furthermore, 61.7% reported lack of appetite, 55.8% indigestion, 57% nausea and vomiting, 55.5% food regurgitation, and 41.5% chest discomfort as an associated symptom. The primary risk factors discovered were coffee consumption in 77.5%, spicy food in 58.0%, smoking in 17.1% fatty meals in 84.8%, NSAD in 24.5%, and stress in 71% participants. There was no significant difference in the occurrence of GERD based on educational level, age, employment status, marital status, or gender. Conclusion: The percentage of gastroesophageal reflux disease (61.7 percent) was extremely high. Spicy foods, coffee use, fatty meals, smoking and stress were identified as factors that increased risk. Endoscopic investigations on a population basis are advised. Keywords: gastroesophageal reflux disease, adults, spicy food, prevalence
The coronavirus disease 19 (COVID-19) is a global pandemic of the twenty-first century and currently fourthwave is creating fear and panic worldwide. It is caused by severe acute respiratory syndrome coronavirus 2(SARS-COV-2), a highly contagious viral infection of humans. The COVID-19 can be spread mainly throughrespiratory droplet particles and in contact with a COVID-19 infected person. Clinical manifestation of COVID-19patients includes cough, fever, diarrhea, loss of taste and smell. In critical cases of COVID-19, the developmentof pneumonia and dyspnea leads to acute respiratory distress syndrome that may cause the death of thepatient. It is well established that Angiotensin-Converting Enzyme 2 (ACE2) receptors on alveolar cells act as anentry gate for the SARS-COV-2. However, ACE2 is also highly expressed in multiple extrapulmonary vital organssuch as the gastrointestinal system, cardiovascular system, kidney, etc. Therefore, the direct viral entry in theseorgans can be a likely pathway of injury. In addition, decoupling of immune responses leads to the cytokinesstorm, which might contribute to the injury of extrapulmonary organs. In this review, we report the multipleorgan pathogenesis and clinical manifestations of COVID-19 patients, which could aid clinicians and researchersin prioritizing therapeutics remedies and developing research for all vital body systems involved.
Aim: To assess the patient Adherence and Elimination of Helicobacter pylori Infection with Once-Daily Triple Therapy versus Traditional Triple Therapy Study design: A longitudinal study Place and Duration: This study was conducted at Pir Abdul Qadir Shah Jeelani Institute of Medical Sciences Gambat Pakistan from Faburay 2020 to February 2021. Methodology: A total of 159 patients with aggressive peptic ulcer disease were checked positive for rapid urease exam or 13C-UBT had been provided either once-daily (Tinidazole 1000 milligrams + Azithromycin 500 milligrams +Rabeprazole 40 milligrams) or twice-daily (Amoxicillin 1000 milligrams +Clarithromycin 500 milligrams +Esomeprazole 40). The fast urease exams with 13C-UBT were performed ten weeks following the end of the medication. Pill counting, standardized surveys, and interviews were used to assess compliance and side effects. Results: When compared to the twice-daily cohort, patient adherence was shown to be higher in the once-daily cohort (84.8 percent) (68.8 percent) respectively. Meanwhile, 74.6 percent of the participants tested negative for H. pylori following therapy with the once-daily prescription and reported symptomatic improvement. The standard regime, on the other hand, resulted in 68.7% of the individuals testing negative to UBT and symptomatic alleviation (p=0.4063). Conclusion: No statistically substantial variation in elimination frequencies between the two regimes was observed. The once-daily prescription, on the other hand, had improved drug adherence than the standard treatment plan, indicating that it could be a secure and a superior tolerated substitute to traditional triple treatments, particularly for patients who do not adhere to the regimen. To corroborate the findings, more research should be done. Keywords: peptic ulcer disease, adults, helicobacter pylori, azithromycin
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