Herbicides have been extensively used globally, resulting in severe environmental pollution. Novel butachlor-degrading Pseudomonas sp. strain But2 isolated from soil can degrade butachlor regardless of the concentration and grows without a lag phase. Specific degradation was increased at 0.01–0.1 mM, and did not change significantly at higher concentrations. During degradation, 2-chloro-N-(2,6-diethylphenyl) acetamide, 2,6-diethylaniline, and 1,3-diethylbenzene were formed, which indicated that deamination occurred. Moreover, Pseudomonas sp. strains could tolerate propanil at up to 0.8 mM. The mixed bacterial culture of Pseudomonas sp. But2 and Acinetobacter baumannii DT (a propanil-degrading bacterial strain) showed highly effective biodegradation of both butachlor and propanil in liquid media and soil. For example, under treatment with the mixed culture, the half-lives of propanil and butachlor were 1 and 5 days, respectively, whereas those for the control were 3 and 15 days. The adjuvants present in herbicides reduced degradation in liquid media, but did not influence herbicide removal from the soil. The results showed that the mixed bacteria culture is a good candidate for the removal of butachlor and propanil from contaminated soils.
In this paper, curcumin nano-emulsions were successfully prepared by combining hominization and ultra-sonication methods which have been mentioned. The optimal conditions for the hominization method have been established as follows: 3% emulsifier concentration, 20,000 rpm of capacity in 60 minutes, the average size of nano drops was 78 nm. Meanwhile, the optimal conditions of the ultrasonic method are also constructed as follows: 2% emulsifier concentration, 450 w/g of ultrasonic power, 20 kHz frequency, the average size of the droplet was 58 nm. Nano-emulsion system has been stable after 4 months of cold storage.
The use of plant-deriveded natural coagulants in water treatment systems is seen as solution to replace chemical coagulants. Among them, the Moringa oleifera has proved to be one of the most effective natural, low - cost, environmentally friendly water coagulants. In this study, Moringa oleifera has used from 0 g (control sample without coagulants) to 2.0 g of Moringa oleifera seed powder in one liter of the Tien river water sample, coagulation time in 1 hour, 3 hours, 6 hours. The results of the study showed that Moringa oleifera seed powder has antibacterial ability when adding 0.4 g per liter of water treated. The water sample has TSS of 93.3 mg/l, COD of 101 mg/l, and the presence of 1530 MPN/100ml Coliform bacteria. After one hour coagulation of treatment, it reduced to TSS, COD, and Coliform were found to be 13.3 mg/l, 18.7 mg/l, and 9 MPN/100ml, respectively. The efficiency of reduction for 76.92% TSS, 81.57% COD, and 99.41% Coliform.
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