In the present research work we evaluate the in vivo distribution of gold nanoparticles (AuNPs) at different time durations after intravenous administration in mice. AuNPs with size of about 20 nm and concentration of 1 mM were synthesized by gamma irradiation method using 0.5% alginate as a stabilizer. AuNPs were characterized by UV-Vis spectrum and transmission electron microscope (TEM) image. The as-synthesized AuNPs solution was centrifuged to concentrate to 2 mg AuNPs/1 ml solution. Intravenous administration of AuNPs in mice was done at the tail with 1 mg AuNPs (0.5 ml). After 1, 3, 6 and 12 h of injection, blood was collected, mice were sacrificed and various tissues/organs were removed. The blood haematology and serum clinical chemistry indexes of mice intravenously injected with AuNPs were not significantly different compared to those of the control ones. In addition, gold content in the samples was quantitatively determined by k 0 -neutron activation analysis (k 0 -NAA) at nuclear research reactor, Da Lat Vietnam. Results showed that after 1 h of administration, AuNPs were mainly accumulated in blood (41.56%), in liver (51.60.%), in lung (6.16%) and in kidney (0.53%). After that the content of AuNPs in blood was decreased to nearly normal at 6 h while the content of AuNPs in liver, lung and kidney was accumulatively increased. After 6 h of administration AuNPs were mainly accumulated in organs like liver (76.33%), lung (11.86%) and kidney (2.23%). Thus, the obtained results are practically useful for using AuNPs as x-ray contrast agent, especially for blood and liver.
The β-glucan extracted from the yeast cell wall was irradiated by gamma rays from a Co-60 source at doses from 100 to 300kGy in swelling condition of 10% (w/v) for degradation. The water soluble content of irradiated sample increased from 25.89 to 66.71% by the increasing of doses from 100 to 300kGy. While the molecular weight of water soluble β-glucan was found to be decreased from 48.13 (at 100kGy) to 10.77 kDa (at 300kGy). The UV-spectra of resultant water soluble β-glucan were appeared a new peak at 265nm with the intensity increased by the increase of the dose. The IR spectra of irradiated β-glucan samples were also recognized by an increase of peak intensity at1731cm-1 assigned to C=O linkages and a decrease of peak intensity at 1156cm-1 assigned to C-O-C glycosidic linkages by the increasing of dose. In addition, the oligoβ-glucan with Mw about 18 kDa obtained at 250kGy-irradiated sample strongly promoted the growth of mustard green and lettuce. The oligoβ-glucan prepared by radiation techniques showed as a high effective plant growth promoter. This product is potentially promising for application in high-technological agriculture for production of clean vegetables and agro-products.
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