M cells are a kind of intestinal epithelial cell in the follicle-associated epithelium of Peyer's patches. These cells can transport antigens and microorganisms into underlying lymphoid tissues. Despite the important role of M cells in mucosal immune responses, the origin and mechanisms of differentiation as well as cell death of M cells remain unclear. To clarify the mechanism of M cell differentiation, we established a novel murine intestinal epithelial cell line (MIE) from the C57BL/6 mouse. MIE cells grow rapidly and have a cobblestone morphology, which is a typical feature of intestinal epithelial cells. Additionally, they express cytokeratin, villin, cell-cell junctional proteins, and alkaline phosphatase activity and can form microvilli. Their expression of Musashi-1 antigen indicates that they may be close to intestinal stem cells or transit-amplifying cells. MIE cells are able to differentiate into the M cell lineage following coculture with intestinal lymphocytes, but not with Peyer's patch lymphocytes (PPL). However, PPL costimulated with anti-CD3/CD28 MAbs caused MIE cells to display typical features of M cells, such as transcytosis activity, the disorganization of microvilli, and the expression of M cell markers. This transcytosis activity of MIE cells was not induced by T cells isolated from PPL costimulated with the same MAbs and was reduced by the depletion of the T cell population from PPL. A mixture of T cells treated with MAbs and B cells both from PPL led MIE cells to differentiate into M cells. We report here that MIE cells have the potential ability to differentiate into M cells and that this differentiation required activated T cells and B cells.
The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health. Holstein-Friesian cows in early-to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets. The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively). Four experimental periods were used, each consisting of a 21-d adaptation period and 7 d of sampling. Dry matter intake and milk yield were both affected by the type of straw included in the diet. A 1.6 kg/d higher DMI was seen when NIS was fed compared with untreated straw, resulting in a 1.7 kg/d higher milk yield. Milk protein concentration was affected by straw type and starch level, and it was 4 and 3% higher when NIS and high-starch diets were fed, respectively. Diets with NIS were more positively effective when fed with low levels of starch. These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
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